PMID: 8963726May 1, 1996Paper

Endothelial cell tyrosine kinase receptor and G protein-coupled receptor activation involves distinct protein kinase C isoforms

Arteriosclerosis, Thrombosis, and Vascular Biology
H HallerF C Luft

Abstract

Protein kinase C (PKC) is a family of serine/threonine protein kinase isoforms that is important to intracellular enzymes for both tyrosine kinase receptors and G protein coupled receptors. However, which isoforms are linked to which class of receptors in endothelial cell signaling is not known. Moreover, the PKC isoforms in endothelial cells have not been thoroughly characterized. We tested the hypothesis that specific PKC isoforms are involved in different signaling pathways. PKC isoform expression was assessed by using reverse transcription polymerase chain reaction and Western blotting. The spatial distribution of PKC after stimulation of the cells with basic fibroblast growth factor (bFGF) and thrombin was examined by using confocal microscopy. Expression of PKC alpha, delta, epsilon, theta, and zeta was detectable on both the mRNA and protein levels. In resting cells, PKC alpha and epsilon were mostly distributed in the cytosol, while PKC alpha and epsilon were also present in the nucleus. Nuclear immunoreactivity of PKC alpha and epsilon increased significantly between passages 1 and 3. The phorbol ester TPA induced a rearrangement of PKC delta and a translocation of PKC alpha and epsilon to the nucleus. Treatment of end...Continue Reading

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Citations

Jan 18, 2014·Biochemical and Biophysical Research Communications·Hyun Beom SongJeong Hun Kim
Feb 21, 2004·European Journal of Pharmacology·Ken ShimamuraToshihiko Murayama
Dec 15, 2000·Acta Physiologica Scandinavica·A S Battistella-PattersonG L Wright
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Aug 1, 1996·Kidney International·H HallerF C Luft

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