Endothelial cells and endothelin‑1 promote the odontogenic differentiation of dental pulp stem cells

Molecular Medicine Reports
Mingyue LiuXiaofeng Wang

Abstract

It has been established that dental pulp stem cells (DPSCs) serve an important role in the restoration and regeneration of dental tissues. DPSCs are present in blood vessels and also exist in the vessel microenvironment in vivo and have a close association with endothelial cells (ECs). The present study aimed to evaluate the influence of ECs and their secretory product endothelin‑1 (ET‑1) on the differentiation of DPSCs. In the present study, cells were divided into four groups: i) a DPSC‑only control group; ii) a DPSC with ET‑1 administration group; iii) a DPSC and human umbilical vein endothelial cell (HUVEC) direct co‑culture group; and iv) a DPSC and HUVEC indirect co‑culture group using a Transwell system. Reverse transcription‑quantitative polymerase chain reaction was used to detect the expression of the odontoblastic differentiation‑associated genes, including dentin sialoprotein (DSP) and dentin matrix acidic phosphoprotein 1 (DMP‑1) at days 4, 7, 14 and 21. Alizarin Red S staining, immunofluorescence and western blot analyses were also conducted to assess the differentiation of the DPSCs in each group. The highest expression levels of odontoblastic differentiation‑associated genes were observed on day 7 and in the two...Continue Reading

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Citations

Oct 10, 2020·World Journal of Stem Cells·Cornelia Larissa Granz, Ali Gorji
Jul 10, 2021·Frontiers in Cell and Developmental Biology·Bo LiYi Men

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Methods Mentioned

BETA
ELISA
protein assay
PCR
scraping

Software Mentioned

SPSS

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