Endothelial cells are not required for specification of respiratory progenitors

Developmental Biology
Jamie A HavrilakJ M Shannon

Abstract

Crosstalk between mesenchymal and epithelial cells influences organogenesis in multiple tissues, such as lung, pancreas, liver, and the nervous system. Lung mesenchyme comprises multiple cell types, however, and precise identification of the mesenchymal cell type(s) that drives early events in lung development remains unknown. Endothelial cells have been shown to be required for some aspects of lung epithelial patterning, lung stem cell differentiation, and regeneration after injury. Furthermore, endothelial cells are involved in early liver and pancreas development. From these observations we hypothesized that endothelial cells might also be required for early specification of the respiratory field and subsequent lung bud initiation. We first blocked VEGF signaling in E8.5 cultured foreguts with small molecule VEGFR inhibitors and found that lung specification and bud formation were unaltered. However, when we examined E9.5 mouse embryos carrying a mutation in the VEGFR Flk-1, which do not develop endothelial cells, we found that respiratory progenitor specification was impeded. Because the E9.5 embryos were substantially smaller than control littermates, suggesting the possibility of developmental delay, we isolated and cultu...Continue Reading

Citations

Nov 15, 2018·Physiological Reviews·Jeffrey A WhitsettVladimir V Kalinichenko
Nov 30, 2019·Frontiers in Bioengineering and Biotechnology·Akiko Mammoto, Tadanori Mammoto
May 29, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·James P BridgesYan Xu
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Yelda Pakize KinaElie El Agha
Jun 20, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Oliver A StoneDidier Y R Stainier

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Methods Mentioned

BETA
dissection
antisense oligodeoxynucleotides
PCR

Software Mentioned

Bitplane Imaris

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