Endothelial cells do not express GSTA1: potential relevance to busulfan-mediated endothelial damage during haematopoietic stem cell transplantation

European Journal of Haematology
Camille VassordRajagopal Krishnamoorthy

Abstract

Busulfan-mediated endothelial damage is believed to be a common mechanism in a variety of vascular disorders that occur during haematopoietic stem cell transplantation. The alkylating capacity of busulfan is compromised in vivo by enzymatic conjugation with glutathione, principally catalysed by glutathione S-transferase alpha (GST alpha). We investigated whether the susceptibility of endothelial cells to busulfan-mediated damage is related to their intrinsic deficiency in GST alpha expression. We tested for the expression of GST alpha mRNA by real-time quantitative PCR and the GST protein by enzyme-linked immunosorbent assay in various independently derived endothelial cell types (human bone marrow-derived endothelial cell line and endothelial cells from human vein umbilical cord ) and in a control hepatic cell line, HepG2. We demonstrate that endothelial cells, contrary to hepatic cells do not express GST alpha either at mRNA or protein levels and hence are potentially susceptible to busulfan-mediated cytotoxic damage.

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Citations

Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Abdolamir AllamehMasoud Soleimani
Apr 27, 2012·Journal of Diabetes and Its Complications·Peter Holmquist, Petru Liuba
Feb 22, 2012·Metabolic Brain Disease·Krisztián KállayGergely Kriván
Nov 1, 2020·Clinical Pharmacokinetics·Rachael LawsonStefanie Hennig

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