Endothelial cells exhibit differential chemokinetic and mitogenic responsiveness to alpha-thrombin

The Journal of Surgical Research
H S WangE L Chaikof

Abstract

Rapid regeneration of the endothelium is a critical component of vascular wall repair because limitations of this process enhance early thrombotic and vasospastic complications, as well as late sequelae of recurrent lesion formation. We have postulated that direct activation of the thrombin receptor initiates both mitogenic and chemokinetic endothelial behavior which facilitates intimal repair. To characterize the role of the thrombin receptor in human endothelial cell (EC) proliferation and migration, we investigated the effects of both alpha-thrombin (0.5-10 U/ml) and its receptor-activating peptide (TRAP; 1-100 microM). Responses of human aortic (HAEC) and umbilical vein (HUVEC) were characterized using [3H]thymidine and 61Cr microcarrier bead assays of proliferation and migration, respectively. Expression of motility-related genes was evaluated using a ribonuclease protection assay. Thrombin exerts both of its chemokinetic and mitogenic effects differentially in human endothelial cells. Following 2 or 4 days in culture, HUVEC proliferation increased two- to threefold after exposure to thrombin, primarily in the low concentration range (P < 0.05). However, HACE proliferation was inhibited up to 50% after a 4-day incubation p...Continue Reading

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Citations

Mar 4, 2005·The American Journal of Pathology·Shu Jun SongEleanor J Mackie
Oct 26, 2016·Journal of Vascular Surgery·Rohun U PalekarSamuel A Wickline
Jun 17, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Martin Moser, Cam Patterson
Jul 8, 2005·Cell Motility and the Cytoskeleton·Tatiana M VinogradovaVictoria B Bystrevskaya
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A M HealyH W Farber
Jan 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Mano R MauryaShankar Subramaniam
Jun 27, 2003·Microvascular Research·Barden ChanVikas P Sukhatme

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