Endothelial cells from cord blood CD133+CD34+ progenitors share phenotypic, functional and gene expression profile similarities with lymphatics.

Journal of Cellular and Molecular Medicine
Van Anh NguyenNorbert Sepp

Abstract

The existence of endothelial progenitor cells (EPC) with high cell-cycle rate in human umbilical cord blood has been recently shown and represents a challenging strategy for therapeutic neovascularization. To enhance knowledge for future cellular therapy, we compared the phenotypic, functional and gene expression differences between EPC-derived cells generated from cord blood CD34(+) cells, and lymphatic and macrovascular endothelial cells (EC) isolated from human foreskins and umbilical veins, respectively. Under appropriate culture conditions, EPC developed into fully matured EC with expression of similar endothelial markers as lymphatic and macrovascular EC, including CD31, CD36, von Willebrand factor FVIII, CD54 (ICAM-1), CD105 (endoglin), CD144 (VE-cadherin), Tie-1, Tie-2, VEGFR-1/Flt-1 and VEGFR-2/Flk-1. Few EPC-derived cells became positive for LYVE-1, indicating their origin from haematopoietic stem cells. However they lacked expression of other lymphatic cell-specific markers such as podoplanin and Prox-1. Functional tests demonstrated that the cobblestone EPC-derived cells up-regulated CD54 and CD62E expression in response to TNF-alpha, incorporated DiI-acetylated low-density liproprotein and formed cord- and tubular-...Continue Reading

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Citations

Jul 27, 2011·Surgical and Radiologic Anatomy : SRA·Shin-ichi AbeYoshinobu Ide
Jan 25, 2011·Antioxidants & Redox Signaling·Cantas AlevTakayuki Asahara
Jan 25, 2013·Tissue Engineering. Part a·Cristian D ValenzuelaStephen M Warren
Jan 16, 2010·Tissue Engineering. Part C, Methods·Lee-Lee OngGustav Steinhoff
Nov 26, 2015·Experimental Cell Research·Terri A DiMaioMichael Lagunoff
Jun 16, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Jeffrey M BarrettClaudine S Bonder
Jan 24, 2014·Journal of Cellular and Molecular Medicine·Yu-zhen TanQi-zhi He
Oct 21, 2014·Stem Cells and Development·Shuqun Qi, Jian Pan

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
chip
light microscopy
FACS

Software Mentioned

Bioconductor

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