PMID: 2493194Feb 1, 1989Paper

Endothelial cells from human umbilical vein secrete functional transcobalamin II

The American Journal of Physiology
Edward V QuadrosE A Jaffe

Abstract

Transcobalamin II (TCII) is a cobalamin (Cbl) binding protein in the plasma that mediates the cellular uptake of Cbl. Although the synthesis of TCII by a variety of cultured mammalian cells and by some isolated perfused organs has been reported, no single tissue has been identified as the source of TCII in vivo. In this study, we demonstrate that cultured human umbilical vein endothelial cells secrete a protein that binds CN[57Co]Cbl, elutes from a Sephacryl S-200 column in the same position as TCII, and precipitates with an antiserum to purified human TCII. The biosynthesis of TCII by these cells was confirmed by demonstrating the incorporation of [35S]methionine into a nascent protein that immunoprecipitated with anti-TCII and which, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) had an Mr of 43,000, the same as human TCII. This secreted protein also had the functional properties of TCII because it facilitated the uptake of CN[57Co]Cbl by the same endothelial cells that secreted it as well as other cell lines that express the membrane receptor for TCII. We also present evidence that the venous endothelium could be the source of TCII in vivo by showing that an intact umbilical vein in an isolated umbil...Continue Reading

References

Jun 1, 1975·Archives of Biochemistry and Biophysics·P M DiGirolamo, F M Huennekens

❮ Previous
Next ❯

Citations

Jan 1, 1996·Critical Reviews in Clinical Laboratory Sciences·H V Markle
Oct 17, 2009·British Journal of Haematology·Edward V Quadros
May 10, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luciana HannibalDonald W Jacobsen
Jul 17, 1999·The American Journal of Physiology·E V QuadrosS P Rothenberg
Nov 25, 2003·British Journal of Haematology·Fares NamourGuéant Jean-Louis
Oct 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kaveri AroraEdward V Quadros
Dec 6, 2016·Nutrients·Gianluca RizzoSalvatore Giovanni Vitale
Jan 15, 2020·BMC Medical Genetics·Suelen Porto BasgaluppIda Vanessa Doederlein Schwartz
Sep 9, 2020·Experimental Cell Research·Gregory G GickEdward V Quadros
Apr 22, 2021·Critical Reviews in Clinical Laboratory Sciences·Agata Sobczyńska-MaleforaDominic J Harrington
Jun 18, 2002·Blood Cells, Molecules & Diseases·Lian QianSheldon P Rothenberg
Mar 8, 2003·Archives of Biochemistry and Biophysics·Sravan K VanamalaBellur Seetharam
Mar 1, 1994·Journal of the Neurological Sciences·J A BegleyR C Chu
Jan 18, 2005·Biochemical and Biophysical Research Communications·Edward V QuadrosJeffrey M Sequeira
Sep 1, 1995·Baillière's Clinical Haematology·S P Rothenberg, E V Quadros
Nov 28, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Susanne H KirschRima Obeid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.