Endothelial cells prevent accumulation of lipid hydroperoxides in low-density lipoprotein

Arteriosclerosis, Thrombosis, and Vascular Biology
D M SmalleyK A Pritchard

Abstract

A variety of cell types, including endothelial cells, oxidize low-density lipoprotein (LDL). To investigate the mechanisms by which endothelial cells modulate LDL oxidation states, endothelial cell cultures were incubated with LDL (240 mg cholesterol/dL) for 24 hours in M199 supplemented with fetal bovine serum (FBS, 16.7%). These conditions were not toxic to endothelial cells over the time frame of the study. Changes in LDL oxidation were monitored by measuring thiobarbituric acid-reactive substances (TBARS), lipid hydroperoxide (LOOH), and conjugated dienes (A234nm). LDL medium incubated in the absence of endothelial cells contained higher TBARS than did LDL medium incubated with endothelial cells (0.35 +/- 0.08 versus 0.23 +/- 0.08 nmol MDA/mg, respectively). LOOHs were higher in LDL medium incubated without endothelial cells than in LDL medium incubated with endothelial cells (6.8 +/- 4.4 versus 0.49 +/- 0.89 nmol/mg, respectively). Conjugated diene formation, based on changes in absorbance at 234 nm, increased to a greater extent in LDL medium incubated in the absence of endothelial cells than when endothelial cells were present. To increase oxidative stress on the endothelial cell cultures, increasing concentrations of Cu...Continue Reading

References

Jun 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·T HenriksenB Carlander
Jan 1, 1992·Annual Review of Medicine·S ParthasarathyJ L Witztum
Aug 1, 1991·Archives of Biochemistry and Biophysics·R RadiB A Freeman
Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
Jan 11, 1991·The American Journal of Physiology·K A PritchardM B Stemerman
Jul 1, 1990·The Journal of Clinical Investigation·M A CreagerV J Dzau
Dec 1, 1990·The Journal of Clinical Investigation·R L MinorD G Harrison
May 22, 1990·Biochimica Et Biophysica Acta·S ParthasarathyL G Fong
Mar 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·P A WinesW B Campbell
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum
Feb 20, 1986·The New England Journal of Medicine·R Ross
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M E Haberland, A M Fogelman
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S ParthasarathyD Steinberg
Jan 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M B StemermanR S Lees
Jul 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·D W MorelG M Chisolm
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·U P SteinbrecherD Steinberg
Jan 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M B Stemerman
Jan 1, 1995·Advances in Experimental Medicine and Biology·D Steinberg
Feb 23, 1995·The New England Journal of Medicine·G N LevineJ A Vita
Dec 1, 1994·Archives of Biochemistry and Biophysics·J P ThomasA W Girotti
Jun 1, 1993·The Journal of Clinical Investigation·Y OharaD G Harrison
May 28, 1993·Annals of the New York Academy of Sciences·W A Pryor, K Stone
Jan 1, 1996·Free Radical Biology & Medicine·J A Berliner, J W Heinecke
Jan 17, 1997·The Journal of Biological Chemistry·C LeeuwenburghJ W Heinecke

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Citations

Jun 16, 2001·British Journal of Pharmacology·S F O'BrienJ C Russell
Mar 5, 2005·Lipids in Health and Disease·Lillemor Mattsson HulténOlov Wiklund
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anatol KontushM John Chapman
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S H AudiC A Dawson
Jan 15, 2000·The Journal of Biological Chemistry·A BaoutinaW Jessup
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·David W SteppKirkwood A Pritchard

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