Endothelial Glycocalyx Layer Properties and Its Ability to Limit Leukocyte Adhesion.

Biophysical Journal
Luis F DelgadilloRichard E Waugh

Abstract

The endothelial glycocalyx layer (EGL), which consists of long proteoglycans protruding from the endothelium, acts as a regulator of inflammation by preventing leukocyte engagement with adhesion molecules on the endothelial surface. The amount of resistance to adhesive events the EGL provides is the result of two properties: EGL thickness and stiffness. To determine these, we used an atomic force microscope to indent the surfaces of cultured endothelial cells with a glass bead and evaluated two different approaches for interpreting the resulting force-indentation curves. In one, we treat the EGL as a molecular brush, and in the other, we treat it as a thin elastic layer on an elastic half-space. The latter approach proved more robust in our hands and yielded a thickness of 110 nm and a modulus of 0.025 kPa. Neither value showed significant dependence on indentation rate. The brush model indicated a larger layer thickness (∼350 nm) but tended to result in larger uncertainties in the fitted parameters. The modulus of the endothelial cell was determined to be 3.0-6.5 kPa (1.5-2.5 kPa for the brush model), with a significant increase in modulus with increasing indentation rates. For forces and leukocyte properties in the physiologi...Continue Reading

References

Jun 1, 1992·Biophysical Journal·D Needham, R M Hochmuth
Oct 3, 2001·Physical Review Letters·B FabryJ J Fredberg
Apr 20, 2002·Biophysical Journal·Emilios K DimitriadisRichard S Chadwick
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·A W Mulivor, H H Lipowsky
Mar 15, 2003·Circulation Research·Bernard M van den BergJos A E Spaan
Nov 13, 2003·Physical Review Letters·A W C LauT C Lubensky
Jan 30, 2004·Biophysical Journal·Elena B Lomakina, Richard E Waugh
Aug 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Michael B Kim, Ingrid H Sarelius
Sep 14, 2004·Biophysical Journal·E B LomakinaR E Waugh
Sep 14, 2004·Biophysical Journal·C M SpillmannR E Waugh
Oct 19, 2004·Biophysical Journal·Devrim Pesen, Jan H Hoh
Jun 7, 2005·Nature Materials·Predrag BursacJeffrey J Fredberg
Feb 16, 2006·Osteoarthritis and Cartilage·E M DarlingF Guilak
Mar 10, 2006·Journal of Biomechanical Engineering·Kevin D CostaFrank C-P Yin
Mar 22, 2007·Annual Review of Biomedical Engineering·Sheldon WeinbaumEdward R Damiano
Jul 8, 2009·Biophysical Journal·Sandrine A HocdéRichard E Waugh
Jun 28, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ryan O'CallaghanVladimir Hlady
Jul 29, 2011·Pflügers Archiv : European journal of physiology·Hans OberleithnerKilian Oberleithner
Feb 22, 2012·Microvascular Research·Wan-Yi YenBingmei M Fu
Mar 16, 2012·Journal of the Royal Society, Interface·Ke Bai, Wen Wang
Nov 2, 2012·American Journal of Physiology. Cell Physiology·Andrew KooGuillermo García-Cardeña
May 4, 2013·Methods : a Companion to Methods in Enzymology·Igor SokolovNataliia V Guz
Nov 28, 2013·PloS One·Anne WiesingerPhilipp Kümpers
Sep 3, 2014·Macromolecules·Ekaterina B Zhulina, Michael Rubinstein
Oct 2, 2014·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Emad Moeendarbary, Andrew R Harris
Jan 15, 2015·Annals of Biomedical Engineering·Michael T BesteRichard E Waugh
Jan 16, 2016·Microscopy Research and Technique·Hanae KataokaTakehiko Iijima
Aug 16, 2016·American Journal of Physiology. Heart and Circulatory Physiology·William OkechIngrid H Sarelius
Jan 13, 2018·Scientific Reports·Aleksei Kabedev, Vladimir Lobaskin
Feb 10, 2019·Biophysical Journal·Jay G GandhiMatthew J Paszek

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Citations

Apr 3, 2020·Pflügers Archiv : European journal of physiology·Benedikt Fels, Kristina Kusche-Vihrog
Dec 17, 2020·American Journal of Physiology. Cell Physiology·Luis F DelgadilloRichard E Waugh
Jun 1, 2021·Frontiers in Physiology·Carlos Alexandre FalconiMarcela Sorelli Carneiro-Ramos

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