Endothelial hyperpermeability after cardiac surgery with cardiopulmonary bypass as assessed using an in vitro bioassay for endothelial barrier function

British Journal of Anaesthesia
N J KoningC Boer

Abstract

The mechanisms causing increased endothelial permeability after cardiopulmonary bypass (CPB) have not been elucidated. Using a bioassay for endothelial barrier function, we investigated whether endothelial hyperpermeability is associated with alterations in plasma endothelial activation and adhesion markers and can be attenuated by the use of pulsatile flow during CPB. Patients undergoing cardiac surgery were randomized to non-pulsatile (n=20) or pulsatile flow CPB (n=20). Plasma samples were obtained before (pre-CPB) and after CPB (post-CPB), and upon intensive care unit (ICU) arrival. Changes in plasma endothelial activation and adhesion markers were determined by enzyme-linked immunosorbent assay. Using electric cell-substrate impedance sensing of human umbilical vein endothelial monolayers, the effects of plasma exposure on endothelial barrier function were assessed and expressed as resistance. Cardiopulmonary bypass was associated with increased P-selectin, vascular cell adhesion molecule-1, and von Willebrand factor plasma concentrations and an increase in the angiopoietin-2 to angiopoietin-1 ratio, irrespective of the flow profile. Plasma samples obtained after CPB induced loss of endothelial resistance of 21 and 23% in ...Continue Reading

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Citations

Apr 14, 2018·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Richard W PierceJordan S Pober
Jan 14, 2020·Clinical Hemorheology and Microcirculation·Anoek L I van LeeuwenCharissa E van den Brom
Apr 18, 2020·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Jonas HolmZoltán Szabó
Jun 23, 2020·Technology and Health Care : Official Journal of the European Society for Engineering and Medicine·Yi-Ri DuHai-Yan Zhong
Mar 28, 2017·Future Science OA·Emmanuel CharbonneyClaudia Dos Santos
Apr 22, 2020·Proceedings·Bobby Darnell RobinsonClaire Larson Isbell
Apr 20, 2021·Journal of Cardiothoracic and Vascular Anesthesia·Osama Abou-ArabPierre-Grégoire Guinot
Jul 15, 2021·Oxidative Medicine and Cellular Longevity·Petar VukicevicJelena Kotur-Stevuljevic

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