Endothelial injury and leukocyte adherence in Häutchen preparations from coronary arteries and aorta of cynomolgus monkeys

Atherosclerosis
H B SkantzeK Pettersson

Abstract

The aim was to describe the production of en face preparations (Häutchens) of the endothelium from the aorta and the coronary arteries of cynomolgus monkeys (Macaca fascicularis), to describe the morphology of the endothelium and to estimate endothelial injury. The present study describes, for the first time, the application of this technique to the coronary arteries. Numerous leukocytes (peroxidase-positive and -negative cells) were observed on the endothelium and a positive correlation between the numbers of adherent leukocytes and injured endothelial cells was found. A relatively leukocyte-independent measure of endothelial injury in the aorta was obtained by counting areas with a restricted number of leukocytes. The results indicated an even distribution and a relatively low frequency of injured endothelial cells along the arterial tree. The percentage of injured endothelial cells in LAD and LCX was 0.18 and 0.16, and in non-branched and circumostial areas of the descending thoracic aorta the corresponding figures were 0.22 and 0.28. There were, on average, 344 and 236 adherent leukocytes/mm2 in the non-branched and circumostial areas of the descending thoracic aorta, respectively, compared to 13 in the LAD and 23 in the LC...Continue Reading

References

Nov 1, 1975·Atherosclerosis·J B SilkworthW E Stehbens
May 1, 1991·Circulation Research·W B StrawnT B Clarkson
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J Masuda, R Ross
Mar 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S J LinS Chien
Sep 1, 1971·The Journal of Pathology·H P Wright
Jan 1, 1958·The Journal of Pathology and Bacteriology·J C POOLEH W FLOREY

❮ Previous
Next ❯

Citations

Jan 18, 2005·Biochemical and Biophysical Research Communications·Ruo-Zhi ZhaoChangyi Chen
Jan 6, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Andrew R BondPeter D Weinberg
Apr 6, 2006·Palliative & Supportive Care·Karen E SteinhauserJames A Tulsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.