Endothelial isoform of nitric oxide synthase in rat heart increases during development

The Anatomical Record
P C Ursell, M Mayes

Abstract

Although nitric oxide seems important to cardiovascular function, the ontogenesis of this chemical messenger in the heart is unknown. To investigate this issue, we determined the distribution of nitric oxide synthase (NOS) in the rat heart at different stages during development. Immunohistochemistry and the NADPH-diaphorase reaction were used to localize NOS activity-specifically, the endothelial isoform (eNOS) and neuronal isoform (bNOS)-in fetal (14- and 18-day gestation), neonatal (0-, 3-, and 6-day gestation), 12-day-old, 1-month-old, and mature adult Fischer 344 rat hearts. There was sparse eNOS immunoreactivity in the endocardium of the youngest fetal hearts; antibody to eNOS did not localize any vessels within the noncompact myocardium at this stage. By the eighteenth day of embryonic development (e18), eNOS was present not only in endocardium but also in the intima of numerous small vessels within the compact myocardium. In the neonatal rat heart, vascular eNOS predominated, with extensive reaction product in arteries, veins, and numerous capillary size vessels within the myocardium; the endocardium also contained eNOS immunoreactivity. A similar distribution was maintained throughout the remainder of development. Only ...Continue Reading

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Citations

Jun 25, 1998·American Journal of Respiratory Cell and Molecular Biology·Q El DwairiS N Hussain
Sep 4, 1999·Clinical and Experimental Pharmacology & Physiology·D M Kaye, R A Kelly
Jan 5, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sofie Trajanovska, John A Donald
Dec 1, 2012·Free Radical Biology & Medicine·Liliana VieraAlvaro G Estévez
Apr 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bianca C W GroenendijkRobert E Poelmann
Oct 26, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L P ThompsonC P Weiner
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Xue ZhaoQingping Feng

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