Endothelial mediators of the acetylcholine-induced relaxation of the rat femoral artery

Vascular Pharmacology
Hok Sum LeungYu Huang

Abstract

This study examined endothelium-derived mediators of acetylcholine-induced relaxation in male rat femoral arteries. Arterial rings were suspended in a myograph for the measurement of isometric force. The generation of hydrogen peroxide (H2O2) in endothelial cells was detected using the fluorescent probe, 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester. N(G)-nitro-L-arginine methyl ester (L-NAME, NOS inhibitor) and 1H-[1,2,4]oxadiazolo[4,2-alpha]quinoxalin-1-one (ODQ, guanylate cyclase inhibitor) alone or in combination with indomethacin (cycloxygenase inhibitor) diminished acetylcholine-induced endothelium-dependent relaxation to a similar extent. A small relaxation to acetylcholine in 60 mM KCl-constricted rings was abolished by L-NAME. Acetylcholine-induced relaxation was reduced by charybdotoxin plus apamin (intermediate- and small-conductance Ca2+-activated K+ channel blockers, respectively) or by 30 mM KCl. Both ouabain (Na+/K+ ATPase inhibitor) and BaCl2 (K(IR) channel blocker) also inhibited the relaxation albeit to a lesser degree. In the presence of L-NAME, ODQ plus indomethacin, charybdotoxin plus apamin or ouabain plus BaCl2 produced further inhibition. Catalase attenuated acetylcholine...Continue Reading

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Citations

Jun 12, 2013·Pflügers Archiv : European journal of physiology·David C EllinsworthShaun L Sandow
Sep 5, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·De-Li DongWen-Hui Wang
Feb 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Adrian GerickeNorbert Pfeiffer
Jan 30, 2013·Environmental Health Perspectives·Jose R Suarez-LopezBruce H Alexander
Nov 17, 2009·Microvascular Research·Xia LiShen-Jiang Hu
Nov 23, 2006·Experimental Gerontology·Carlos F Reyes-TosoDaniel P Cardinali
Oct 13, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·J BelikM Letarte
Oct 25, 2017·Journal of Vascular Research·Dominga LapiAntonio Colantuoni
Jul 21, 2020·Cardio-Oncology·Hubert DabiréBijan Ghaleh

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