Endothelial mineralocorticoid receptor ablation does not alter blood pressure, kidney function or renal vessel contractility

PloS One
Sidsel B LaursenHenrik Dimke

Abstract

Aldosterone blockade confers substantial cardiovascular and renal protection. The effects of aldosterone on mineralocorticoid receptors (MR) expressed in endothelial cells (EC) within the renal vasculature have not been delineated. We hypothesized that lack of MR in EC may be protective in renal vasculature and examined this by ablating the Nr3c2 gene in endothelial cells (EC-MR) in mice. Blood pressure, heart rate and PAH clearance were measured using indwelling catheters in conscious mice. The role of the MR in EC on contraction and relaxation was investigated in the renal artery and in perfused afferent arterioles. Urinary sodium excretion was determined by use of metabolic cages. EC-MR transgenics had markedly decreased MR expression in isolated aortic endothelial cells as compared to littermates (WT). Blood pressure and effective renal plasma flow at baseline and following AngII infusion was similar between groups. No differences in contraction and relaxation were observed between WT and EC-MR KO in isolated renal arteries during baseline or following 2 or 4 weeks of AngII infusion. The constriction or dilatations of afferent arterioles between genotypes were not different. No changes were found between the groups with res...Continue Reading

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Sep 6, 2019·Current Hypertension Reports·Jessica L Faulkner, Eric J Belin de Chantemèle
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Jul 2, 2021·Molecular and Cellular Endocrinology·Say ViengchareunLaetitia Martinerie

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Methods Mentioned

BETA
transgenic
force measurements
ELISA
PCR

Software Mentioned

LabView
GraphPad Prism

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