Endothelial NOS is main mediator for shear stress-dependent angiogenesis in skeletal muscle after prazosin administration

American Journal of Physiology. Heart and Circulatory Physiology
Oliver BaumAndreas Zakrzewicz

Abstract

The increase of wall shear stress in capillaries by oral administration of the alpha1-adrenergic receptor antagonist prazosin induces angiogenesis in skeletal muscles. Because endothelial nitric oxide synthase (eNOS) is upregulated in response to elevated wall shear stress, we investigated the relevance of eNOS for prazosin-induced angiogenesis in skeletal muscles. Prazosin and/or the NOS inhibitor Nomega-nitro-L-arginine methyl ester (L-NAME) were given to C57BL/6 wild-type mice and eNOS-knockout mice for 14 days. The capillary-to-fiber (C/F) ratio and capillary density (CD; no. of capillaries/mm2) were determined in frozen sections from extensor digitorum longus (EDL) muscles of these mice. Immunoblotting was performed to quantify eNOS expression in endothelial cells isolated from skeletal muscles, whereas VEGF (after precipitation with heparin-agarose) and neuronal NOS (nNOS) concentrations were determined in EDL solubilizates. In EDL muscles of C57BL/6 mice treated for 14 days, the C/F ratio was 28% higher after prazosin administration and 11% higher after prazosin and L-NAME feeding, whereas the CD increased by 21 and 13%, respectively. The C/F ratio was highest after day 4 of prazosin treatment and decreased gradually to ...Continue Reading

References

Dec 8, 1994·Nature·L KobzikJ S Stamler
Jun 17, 1998·The Journal of Clinical Investigation·T MuroharaJ M Isner
Aug 12, 1999·Cellular and Molecular Life Sciences : CMLS·G Maréchal, P Gailly
Sep 14, 1999·The American Journal of Physiology·S S SegalW C Sessa
Oct 12, 1999·The American Journal of Physiology·P C LeeT R Billiar
Apr 6, 2000·Journal of Applied Physiology·T P GavinP D Wagner
Sep 23, 2000·Nature·G D YancopoulosJ Holash
Dec 29, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S KapurA Marette
Jan 12, 2001·Physiological Reviews·J S Stamler, G Meissner
Feb 13, 2001·Cardiovascular Research·S EggintonO Hudlická
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H T YangR L Terjung
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D FukumuraR K Jain
Mar 14, 2001·Journal of Neuro-oncology·M Ziche, L Morbidelli
Apr 12, 2001·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·E J Van Gieson, T C Skalak
May 15, 2001·American Journal of Physiology. Cell Physiology·N Ferrara
Oct 16, 2001·American Journal of Physiology. Cell Physiology·R W GrangeD J Garry
Nov 10, 2001·Cell and Tissue Research·G PlanitzerO Baum
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·P G LloydR L Terjung
May 8, 2002·Circulation·John P Cooke, Douglas W Losordo
Jun 14, 2002·Cardiovascular Research·Saeid Babaei, Duncan J Stewart
Sep 25, 2002·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Andreas ZakrzewiczAxel R Pries
Dec 25, 2002·Microscopy Research and Technique·Hirotake MasudaChristopher K Zarins
Jan 4, 2003·Hypertension·Katsuya AmanoToshiji Iwasaka
Jan 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Pamela G LloydRonald L Terjung
Mar 11, 2003·Antioxidants & Redox Signaling·Józef Dulak, Alicja Józkowicz
Mar 12, 2003·Trends in Cell Biology·Yuval DorEli Keshet
May 3, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Matthew L SpringerHelen M Blau
Oct 24, 2003·Cell and Tissue Research·Valentin DjonovPeter H Burri

❮ Previous
Next ❯

Citations

Sep 1, 2005·Annals of Biomedical Engineering·Shannon K HughesDonald L Elbert
Jul 23, 2005·Current Osteoporosis Reports·Larry J SuvaDavid M Findlay
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jun YuWilliam C Sessa
May 24, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Andrew V BenestDavid O Bates
May 14, 2009·Circulation Journal : Official Journal of the Japanese Circulation Society·Jiro EsakiRyuzo Sakata
Dec 14, 2005·Cell and Tissue Research·Katharina MatschkeOliver Baum
Jun 13, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Satu MänttäriMatti Järvilehto
Dec 31, 2015·Antioxidants & Redox Signaling·Arif Yurdagul, A Wayne Orr
Jan 2, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Renate KoppThorsten Schwerte
Nov 19, 2005·The Journal of Physiology·James L WilliamsStuart Egginton
Jan 13, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Arif HussainStuart Egginton
Dec 12, 2012·Clinical Physiology and Functional Imaging·T GustafssonO Eiken
Jan 30, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Joseph W WraggRoy Bicknell
Jan 24, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Birgitte Hoier, Ylva Hellsten
Nov 12, 2015·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Tara L Haas, Emmanuel Nwadozi
Jul 30, 2011·Placenta·B J KrauseP Casanello
Apr 28, 2016·Archivos de cardiología de México·Iván Rodríguez-NúñezMaría Javiera Saavedra
Jan 25, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Yusuke HirayamaHidemi Fujino
Mar 14, 2014·Journal of Cardiovascular Pharmacology·Tonino StatiGiuseppe Marano
Jul 16, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Oliver BaumI Mark Olfert
Sep 9, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Oliver BaumIris Baumgartner
Dec 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ylva HellstenJens Bangsbo
Feb 12, 2019·Biofabrication·Alisa Morss ClyneAndrés Díaz Lantada
May 1, 2020·Journal of Applied Physiology·Kyoungrae KimBruno T Roseguini
Jul 14, 2020·Exercise and Sport Sciences Reviews·Kyoungrae KimBruno T Roseguini
Aug 22, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Matteo FiorenzaJens Bangsbo
May 4, 2017·Molecular Neurobiology·Karim A Alkadhi
Apr 12, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Oliver BaumHans Hoppeler
Feb 19, 2019·Journal of Alzheimer's Disease : JAD·Kieran J MarstonJeremiah J Peiffer
Oct 30, 2014·American Journal of Physiology. Endocrinology and Metabolism·Thorbjorn AkerstromYlva Hellsten
Nov 28, 2018·The Journal of Physiological Sciences : JPS·Tomohiro MatsumotoHidemi Fujino

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