Endothelial Progenitor Cell Migration-Enhancing Factors in the Secretome of Placental-Derived Mesenchymal Stem Cells

Stem Cells International
Witchayaporn KampromSurapol Issaragrisil

Abstract

Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. MSCs derived from placental tissues (PL-MSCs) significantly enhanced EPC migration while BM-MSCs, which are the standard source of MSCs for various clinical applications, did not. By using protein fractionation and mass spectrometry analysis, we identified several novel candidates for EPC migration enhancing factor in PL-MSCs secretome that could be used to enhance neovascularization in the injured/ischemic tissues. We recommend that the strategy developed in our study could be used to systematically identify therapeutically useful molecules in the secretomes of other MSC sources for the clinical applications.

References

Jun 27, 2002·Seminars in Cancer Biology·Manuel PatarroyoIsmo Virtanen
Dec 8, 2004·Stem Cells·Pieternella S In 't AnkerHumphrey H H Kanhai
Jun 15, 2007·Molecular & Cellular Proteomics : MCP·Siu Kwan SzeSai-Kiang Lim
Nov 13, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Niranjan AwasthiRoderich E Schwarz
Nov 26, 2008·Circulation Research·Massimiliano GnecchiVictor J Dzau
Jan 28, 2009·Nature Reviews. Immunology·Antonio UccelliVito Pistoia
Apr 8, 2009·The Journal of Biological Chemistry·Omer F HatipogluYoshifumi Ninomiya
Nov 21, 2009·Cytokine & Growth Factor Reviews·Lindolfo da Silva MeirellesArnold I Caplan
Sep 28, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Hongxia LiMeifeng Xu
Mar 23, 2011·Stem Cell Research·Adam OskowitzRadhika Pochampally
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Mar 6, 2012·Cell Stem Cell·Sudhir H RanganathJeffrey M Karp
Apr 30, 2013·Methods in Molecular Biology·Valeria SeverinoAngela Chambery
Jun 15, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Rajesh KatarePaolo Madeddu
May 1, 2009·International Journal of Stem Cells·Chae Woon ParkHoeon Kim
Apr 1, 2015·Stem Cells Translational Medicine·Patrizia DanieliMassimiliano Gnecchi

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Methods Mentioned

BETA
bone marrow aspiration
density gradient centrifugation
flow cytometry
phase-contrast microscopy
reverse-phase chromatography
ELISA

Software Mentioned

MASCOT
SecretomeP
SignalP
CellQuest
DeCyder
PANTHER
DeCyder MS differential analysis
UniProt

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