Endothelial progenitor cell number is not decreased in 34 children with Juvenile Dermatomyositis: a pilot study

Pediatric Rheumatology Online Journal
Dong XuLauren M Pachman

Abstract

A pilot study to determine endothelial progenitor cells (EPC) number in children with Juvenile Dermatomyositis (JDM). After obtaining informed consent, the EPC number from 34 fasting children with definite/probable JDM at various stages of therapy-initially untreated, active disease on medication and clinically inactive, off medication-was compared with 13 healthy fasting pediatric controls. The EPC number was determined by fluorescence activated cell sorting (FACS), CD34+/VEGFR2+/CD45dim-, and assessed in conjunction with clinical variables: disease activity scores (DAS), duration of untreated disease (DUD), TNF-α allelic polymorphism (A/G) at the promoter region of -308, number of nailfold capillary end row loop (ERL) and von Willebrand factor antigen (vWF:Ag). Correlations of the EPC numbers with the clinical and demographic variables, including DAS Skin (DAS SK), DAS Weakness (DAS WK), DAS Total Score, DUD, Cholesterol, triglycerides, High-Density Lipoprotein (HDL) and Low-Density Lipoprotein (LDL), and ERL were calculated using the Pearson correlation coefficient. Tests of associations of EPC with gender (boy vs girl), TNF-α-308A allele (GA/AA vs GG), vWF:Ag (categorized by specific ABO type) as normal/abnormal were perfor...Continue Reading

References

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Citations

Aug 3, 2019·The Journal of Rheumatology·Takayuki KishiLisa G Rider
Jul 29, 2021·The American Journal of Cardiology·Lauren N ManningJohn R Charpie

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Methods Mentioned

BETA
fluorescence activated cell sorting
flow cytometry
FACS

Software Mentioned

FlowJo

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