Endothelial progenitor cells express PAF receptor and respond to PAF via Ca(2+)-dependent signaling

Biochimica Et Biophysica Acta
Maria Luisa BalestrieriLuigi Servillo

Abstract

Endothelial progenitor cell (EPC) therapy is a promising approach to promote angiogenesis and endothelial repair in patients with cardiovascular diseases (CVD). However, their release of proinflammatory mediators may compromise the therapeutic efficacy. Little is known about the role of Platelet-Activating Factor (PAF) in EPC functional response. Here, we investigated the expression of PAF receptor (PAF-R) in early EPC and the release of PAF under stimulation with factors involved in endothelial dysfunction. Results indicated that early EPC express the PAF-R and respond to PAF signaling via a transient increase of cytoplasmic Ca(2+) concentration. EPC release PAF in a time dependent manner upon stimulation with tumor necrosis factor-alpha (TNF-alpha) or high-glucose concentration with a peak at 30 min and 10 min (p<0.01 vs. control), respectively. PAF, starting at concentration of 50 ng/ml, exerted a detrimental effect on EPC number with a concomitant increase of p38 activity. Furthermore, both the reduction of early EPC number and the enhanced p38 activity induced by PAF were abolished by CV3988, a PAF receptor antagonist. These novel findings, revealing that early EPC respond to PAF signaling, unveil an inflammatory pathway t...Continue Reading

Citations

Jul 15, 2015·Biomedical Reports·Mingjie ZhouNan Tian
Apr 19, 2015·Journal of Hematology & Oncology·Ya-Feng LiXiao-Feng Yang
Mar 30, 2013·Toxicology Letters·Paola PedataMaria Luisa Balestrieri
Nov 12, 2013·Food Chemistry·Mariarenata SessaRong Tsao
Jan 7, 2014·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Catherine J Hunter, Isabelle G De Plaen
Dec 14, 2011·Biochemical and Biophysical Research Communications·William G PierceJohn J Mackrill
Jul 24, 2014·Journal of Cellular Biochemistry·Ivana SirangeloMaria Luisa Balestrieri
Nov 22, 2012·Journal of Cellular Physiology·Ivana SirangeloMaria Luisa Balestrieri

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