Endothelial progenitor cells regenerate infracted myocardium with neovascularisation development

Journal of Advanced Research
M T Abd El AzizKhaled A Elsetohy

Abstract

We achieved possibility of isolation, characterization human umbilical cord blood endothelial progenitor cells (EPCs), examination potency of EPCs to form new blood vessels and differentiation into cardiomyoctes in canines with acute myocardial infarction (AMI). EPCs were separated and cultured from umbilical cord blood. Their phenotypes were confirmed by uptake of double stains dioctadecyl tetramethylindocarbocyanine-labeled acetylated LDL and FITC-labeled Ulex europaeus agglutinin 1 (DILDL-UEA-1). EPCs of cord blood were counted. Human VEGFR-2 and eNOS from the cultured EPCs were assessed by qPCR. Human EPCs was transplanted intramyocardially in canines with AMI. ECG and cardiac enzymes (CK-MB and Troponin I) were measured to assess severity of cellular damage. Histopathology was done to assess neovascularisation. Immunostaining was done to detect EPCs transdifferentiation into cardiomyocytes in peri-infarct cardiac tissue. qPCR for human genes (hVEGFR-2, and eNOS) was done to assess homing and angiogenic function of transplanted EPCs. Cultured human cord blood exhibited an increased number of EPCs and significant high expression of hVEGFR-2 and eNOS genes in the culture cells. Histopathology showed increased neovascularizati...Continue Reading

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Citations

Dec 8, 2017·Regenerative Medicine·Khawaja Husnain HaiderMateq Ali Al-Reshidi
Feb 1, 2019·The Journal of International Medical Research·Ling-Bing MengTao Gong
Mar 3, 2020·Frontiers in Veterinary Science·Suryo KuncorojaktiChenphop Sawangmake
Nov 17, 2016·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S A ZayedFa Abdel Maksoud

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Methods Mentioned

BETA
density-gradient centrifugation
PCR

Software Mentioned

SPSS
Applied

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