Endothelial progenitor cells-secreted extracellular vesicles containing microRNA-93-5p confer protection against sepsis-induced acute kidney injury via the KDM6B/H3K27me3/TNF-α axis.

Experimental Cell Research
Zhonghua HeLingju Yue

Abstract

The pivotal pathogenetic role of microRNAs (miRs) in sepsis-induced acute kidney injury (AKI) has been demonstrated in mounting evidence. The functions of the target cells are regulated through the release of cells-encapsulated extracellular vesicles (Evs) into the extracellular space. The present study aims to elucidate the clinical significance as well as biological function of the endothelial progenitor cell (EPC)-derived Evs containing miR-93-5p in sepsis-induced AKI. We first established a cellular sepsis-induced AKI mouse model by treatment with lipopolysaccharide (LPS), and tested ectopic expression and depletion experiments in the model. Evs derived from miR-93-5p inhibitor-transfected EPCs (Evs/miR-93-5p inhibitor) were isolated, and co-cultured with HK2 cells to explore the effects of EPC-derived Evs overexpressing miR-93-5p on LPS-induced HK2 cell injury. The interaction between miR-93-5p and lysine (K)-specific demethylase 6B (KDM6B) was identified using dual-luciferase reporter assay, and ChIP was used to validate the relationship between KDM6B and tumor necrosis factor-α (TNF-α). Mice were made septic by cecal ligation and puncture (CLP), and then injected with Ev/miR-93-5p inhibitor to explore its functions in vi...Continue Reading

References

May 14, 2011·Journal of the American Society of Nephrology : JASN·Abolfazl Zarjou, Anupam Agarwal
Feb 15, 2012·Intensive Care Medicine·Sushma K CribbsGreg S Martin
Apr 9, 2014·American Journal of Respiratory and Critical Care Medicine·Hongkuan FanJames A Cook
Jun 14, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Antero SalminenAnu Kauppinen
Jun 17, 2015·PloS One·Elia Aguado-FraileMaría Laura García-Bermejo
Jul 3, 2015·Cold Spring Harbor Perspectives in Biology·Kathryn N Ivey, Deepak Srivastava
Dec 29, 2015·Transplantation Proceedings·X XuH Wang
Jan 9, 2017·Biochemical and Biophysical Research Communications·Shanshan WangHongjun Miao
Mar 24, 2017·Environmental Science and Pollution Research International·Priyanka Prasad, Dona Sinha
Dec 12, 2017·Nature Reviews. Nephrology·Fabienne Venet, Guillaume Monneret
Mar 16, 2018·Critical Care : the Official Journal of the Critical Care Forum·Juliana Monte RealLuciano Cesar Pontes Azevedo
Mar 31, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yue ZhouHongkuan Fan
Feb 15, 2019·Critical Care : the Official Journal of the Critical Care Forum·Yue ZhouHongkuan Fan

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Citations

May 3, 2021·Free Radical Biology & Medicine·Diana Karpman, Ashmita Tontanahal
Jun 3, 2021·International Journal of Molecular Sciences·Raphael Rodrigues CorrêaRafael Soares Lindoso
Jun 18, 2021·International Urology and Nephrology·Jun PengJia-Qing Peng
Jul 3, 2021·International Journal of Molecular Sciences·Sonia TerriacaAugusto Orlandi
Aug 20, 2021·The Journal of International Medical Research·Hai-Peng FanHong Yan
Aug 27, 2021·Frontiers in Cell and Developmental Biology·Bei YinLing Ye
Oct 5, 2021·Frontiers in Medicine·Latthawan MonnamornRaphatphorn Navakanitworakul
Oct 17, 2021·Diabetology & Metabolic Syndrome·Li ZhaoHualing Ruan

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