Endothelial quality of eye bank-prestripped DMEK prepared form organ-cultured corneas with the Muraine technique

Cell and Tissue Banking
Zhiguo HeMarc Muraine

Abstract

Our aim was to measure the endothelial quality of prestripped Descemet membrane endothelial keratoplasty (DMEK) 48 h after preparation in an eye bank with the Muraine technique and shipping in a distant center. Ten pairs of human corneas with similar eye bank endothelial cell density (ebECD) were stored in organ-culture (OC) for 25 days (20, 28) [median (10-90 percentiles)]. One cornea was then randomized to DMEK preparation using the Moria Muraine trephine, the other served as control. The grafts were left attached to the center of the cornea, immersed in the OC medium (without Dextran) and shipped to a distant center. After 48 h, the viable ECD (vECD) was assessed by image analysis after staining with Hoechst/Ethidium/Calcein-AM. In addition, immunostaining was performed on flat mounted tissues for structural (ZO-1, NCAM, CD166) and functional (Na+/K+ ATPase) proteins of ECs, and for collagen I. Just before stripping, ebECD was 2428 (2268-2669) cells/mm2 for DMEK and 2471 (2135-2714) for controls (P = 1). Forty-eight hours after stripping, vECD was 2057 (1829-2463) cells/mm2 for DMEK and 2119 (1496-2525) for controls (P = 0.508). The expression patterns of the 5 proteins were similar in ECs of both groups. Notably, the deep p...Continue Reading

References

Apr 26, 2002·Journal of Cataract and Refractive Surgery·Mohammed Anwar, Klaus D Teichmann
May 7, 2009·Current Opinion in Ophthalmology·Isabel DapenaGerrit R J Melles
Jan 12, 2011·Archives of Ophthalmology·Isabel DapenaGerrit R J Melles
Jun 15, 2011·Investigative Ophthalmology & Visual Science·Aurélien PipparelliMarc Muraine
Aug 13, 2013·American Journal of Ophthalmology·Marc MuraineDavid Toubeau
Oct 23, 2013·Cornea·Francis W Price, Marianne O Price
Feb 12, 2014·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Brian A Nelson, Rusty J Ritenour
Nov 13, 2014·Investigative Ophthalmology & Visual Science·Naoki OkumuraNoriko Koizumi
Dec 6, 2014·Investigative Ophthalmology & Visual Science·Yu-Chi LiuJodhbir S Mehta
Dec 21, 2014·American Journal of Ophthalmology·Sophie X DengLuxia Chen
Dec 4, 2015·JAMA Ophthalmology·Philippe GainGilles Thuret
Jul 7, 2016·Scientific Reports·Zhiguo HeGilles Thuret
Oct 9, 2016·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sonja HeinzelmannPhilip Maier
Dec 15, 2016·The British Journal of Ophthalmology·Anne Sophie GauthierPhilippe Gain
Dec 16, 2016·The British Journal of Ophthalmology·Johannes Menzel-SeveringTheofilos Tourtas
Apr 12, 2017·Cell and Tissue Banking·Clotilde JumelleGilles Thuret
Oct 7, 2017·The British Journal of Ophthalmology·Koji KitazawaShigeru Kinoshita
Nov 15, 2017·The British Journal of Ophthalmology·Vito RomanoHannah J Levis

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Citations

Aug 27, 2019·Critical Reviews in Food Science and Nutrition·Ankita RaiAnurag Tripathi
Aug 1, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·David EvelethRalph A Bradshaw
May 25, 2021·Current Eye Research·Alina MironJacqueline van der Wees
Jul 27, 2021·Clinical & Experimental Ophthalmology·Paul N BairdKarl D Brown

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Methods Mentioned

BETA
dissections
dissection
confocal microscopy

Software Mentioned

CorneaJ
SPSS Statistics
ImageJ
CellSens
image J
QCAPTURE PRO
Image
NCAM
Pro Express

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