Endothelial Regulator of Calcineurin 1 Promotes Barrier Integrity and Modulates Histamine-Induced Barrier Dysfunction in Anaphylaxis

Frontiers in Immunology
Constanza Ballesteros-MartinezVanesa Esteban

Abstract

Anaphylaxis, the most serious and life-threatening allergic reaction, produces the release of inflammatory mediators by mast cells and basophils. Regulator of calcineurin 1 (Rcan1) is a negative regulator of mast-cell degranulation. The action of mediators leads to vasodilation and an increase in vascular permeability, causing great loss of intravascular volume in a short time. Nevertheless, the molecular basis remains unexplored on the vascular level. We investigated Rcan1 expression induced by histamine, platelet-activating factor (PAF), and epinephrine in primary human vein (HV)-/artery (HA)-derived endothelial cells (ECs) and human dermal microvascular ECs (HMVEC-D). Vascular permeability was analyzed in vitro in human ECs with forced Rcan1 expression using Transwell migration assays and in vivo using Rcan1 knockout mice. Histamine, but neither PAF nor epinephrine, induced Rcan1-4 mRNA and protein expression in primary HV-ECs, HA-ECs, and HMVEC-D through histamine receptor 1 (H1R). These effects were prevented by pharmacological inhibition of calcineurin with cyclosporine A. Moreover, intravenous histamine administration increased Rcan1 expression in lung tissues of mice undergoing experimental anaphylaxis. Functional in vi...Continue Reading

References

May 2, 2002·Cell·Gerald R Crabtree, Eric N Olson
Aug 20, 2004·Pharmacological Reviews·Daniel L SimmonsTimothy Hla
Mar 9, 2005·The Journal of Allergy and Clinical Immunology·UNKNOWN Joint Task Force on Practice ParametersUNKNOWN Joint Council of Allergy, Asthma and Immunology
Aug 4, 2006·The Journal of Clinical Investigation·Anje CauwelsPeter Brouckaert
May 12, 2007·Immunology and Allergy Clinics of North America·Simon G A Brown
Jun 28, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kelvin J A DaviesXavier Estivill
Nov 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoyu TanKottarappat N Dileepan
Jan 7, 2009·The Journal of Experimental Medicine·Yong Jun YangTong-Jun Lin
Jan 28, 2009·The Journal of Experimental Medicine·Hanna KorhonenStefan Offermanns
Jul 1, 2009·Expert Reviews in Molecular Medicine·Puneet KumarSarah Y Yuan
May 14, 2010·Cardiovascular Research·Walter N DuránFabiola A Sánchez
May 22, 2010·Seminars in Thrombosis and Hemostasis·Laura A Dyer, Cam Patterson
Dec 21, 2010·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Volker Spindler, Jens Waschke
Mar 1, 2011·Clinical and Experimental Immunology·G Ram, J Chinen
Jun 11, 2011·Current Opinion in Allergy and Clinical Immunology·Bilal Q Khan, Stephen F Kemp
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marat V KhodounFred D Finkelman
Aug 10, 2011·The Journal of Allergy and Clinical Immunology·Liam O'MahonyCezmi A Akdis
Sep 6, 2011·The Journal of Biological Chemistry·Seon Sook Kim, Su Ryeon Seo
Sep 21, 2011·The Journal of Experimental Medicine·Vanesa EstebanJuan Miguel Redondo
Aug 14, 2012·Medicinal Research Reviews·Robert R RigorSarah Y Yuan
Sep 7, 2012·Current Molecular Medicine·M-G PanF Chen
Oct 9, 2012·The Journal of Allergy and Clinical Immunology·Peter VadasGary Liss
Apr 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert D JunkinsTong-Jun Lin
Jun 5, 2013·Scientific Reports·Gyohei EgawaKenji Kabashima
Jun 20, 2013·Journal of Cell Science·Elisabetta Dejana, Fabrizio Orsenigo
Aug 7, 2013·The Journal of Allergy and Clinical Immunology·Simon G A BrownGeoffrey K Isbister
Oct 16, 2013·EMBO Molecular Medicine·Nerea Méndez-BarberoMiguel R Campanero
Nov 13, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhengli WuTong-Jun Lin

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Citations

Nov 6, 2018·EMBO Reports·David RotterBeverly A Rothermel
May 28, 2019·Frontiers in Immunology·Márta L DebreczeniLászló Cervenak
Dec 2, 2020·Cells·Konstantinos Katsoulis-DimitriouAnne Dudeck
Mar 2, 2021·Frontiers in Microbiology·Jizhen WeiShiheng An
May 1, 2021·International Journal of Molecular Sciences·Martin VossAnne Dudeck
Apr 21, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Emilio Nuñez-BorqueVanesa Esteban
Jul 13, 2021·Frontiers in Immunology·Alma Yuste-MontalvoVanesa Esteban
Nov 5, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryan P MendozaJared M Brown

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Methods Mentioned

BETA
PCR
transfection
flow cytometry
biopsies
fluorescence microscopy
transgenic

Software Mentioned

GraphPad Prism

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