Endothelial type nitric oxide synthase in skeletal muscle fibers: mitochondrial relationships

Biochemical and Biophysical Research Communications
L KobzikJ S Stamler

Abstract

Immunohistochemistry shows that skeletal muscle fibers express endothelial-type (ec)-NOS in a heterogeneous pattern distinct from that of neuronal (nc)-NOS. Analysis of adjacent serial sections reveals: 1) a strong correlation of ec-NOS expression to mitochondrial content (visualized histochemically by succinate dehydrogenase); 2) lack of correlation of ec-NOS to fiber ATPase type (in contrast to nc-NOS); and 3) constitutive co-expression of ec- and nc-NOS in some fibers. Preparations of mitochondria from diaphragm exhibited calcium-dependent NOS activity, which functioned to inhibit in vitro oxygen consumption. The results establish that normal skeletal muscle cells can express two constitutive isoforms of NOS (nc- and ec-) and suggest a functional role for ec-NOS in oxidative, mitochondria-rich fibers.

Citations

Oct 1, 1996·Journal of Applied Physiology·S N Hussain
Oct 24, 1997·Journal of Applied Physiology·W J PerkinsG C Sieck
Aug 1, 1997·American Journal of Respiratory Cell and Molecular Biology·S N HussainY Guo
May 12, 1998·The American Journal of Physiology·D J MitchellK Tyml
Apr 8, 1998·The American Journal of Physiology·Q el-DwairiS N Hussain
Jun 25, 1998·American Journal of Respiratory Cell and Molecular Biology·Q El DwairiS N Hussain
Aug 27, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J BoczkowskiJ J Poderoso
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·A S ComtoisS N Hussain
Apr 25, 2000·Clinical and Experimental Pharmacology & Physiology·B A Kingwell
Feb 16, 2000·The Tohoku Journal of Experimental Medicine·Y TakahashiC Shindoh
Aug 24, 2000·Journal of Applied Physiology·A A SteinerL G Branco
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C R Richmonds, H J Kaminski
Jun 13, 2001·Circulation·J P Cooke, P S Tsao
Dec 19, 2001·Microscopy Research and Technique·C L Murrant, M B Reid
Oct 20, 2001·Journal of Applied Physiology·L M HeunksG C Sieck
May 8, 2002·Nature Reviews. Molecular Cell Biology·Salvador Moncada, Jorge D Erusalimsky
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Esther BarreiroSabah N A Hussain
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhiqiang ChenJonathan S Stamler
Feb 1, 2003·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Robert C HicknerMary Ellen Hostetler
Jan 30, 2003·American Journal of Respiratory and Critical Care Medicine·Cristiane RitterSabah N A Hussain
Jul 26, 2003·Antioxidants & Redox Signaling·Alberto BoverisAna Navarro
Apr 8, 2004·The Journal of Biological Chemistry·Matthew W Foster, Jonathan S Stamler
Sep 14, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaoping ZhuP N Richard Dekhuijzen
Nov 4, 2006·Journal of Applied Physiology·Malcolm J JacksonJesus Palomero
Sep 2, 2008·Clinical and Experimental Pharmacology & Physiology·Glenn K McConell, Glenn D Wadley
Mar 8, 2014·British Journal of Pharmacology·B BuzadzicV Otasevic
Jul 31, 2014·Pflügers Archiv : European journal of physiology·Maria Concetta MiniaciPietro Scotto
Dec 19, 2015·Journal of Pineal Research·Huayqui VoltDarío Acuña-Castroviejo
Nov 13, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Gianni PariseMichael J Plyley
May 1, 1998·American Journal of Respiratory and Critical Care Medicine·K M KrauseM B Reid
Aug 1, 1997·Journal of Applied Physiology·S N HussainD Sakkal
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·K L DavisF Murad
Sep 24, 1999·Biological Chemistry·P Ghafourifar, C Richter
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S FrenchR S Balaban
Apr 27, 2002·Annals of the New York Academy of Sciences·Barry Drew, Christiaan Leeuwenburgh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.