Endothelin-1 and cell proliferation in lung organ cultures. Implications for lung allografts

Transplantation
F RicagnaC G McGregor

Abstract

Endothelin-1 (ET-1) is found in bronchoalveolar lavage fluid in patients following lung transplantation. ET-1 causes contraction of isolated pulmonary vessels and bronchi and stimulates proliferation of smooth muscle cells in culture. Therefore, ET-1 could contribute to the smooth muscle hyperplasia and stromal proliferation seen in chronic rejection of lung allografts. Experiments were designed to determine whether (1) ET-1 stimulates proliferation of pulmonary tissue, (2) proliferation is increased in rejecting allotransplanted lungs, (3) endothelin-A receptors mediate the proliferative response, and (4) ET-1 is produced by activated infiltrating immunocompetent cells. Lung organ cultures were prepared from unoperated dogs and dogs with rejecting single lung allografts. Incubation of organ cultures from unoperated dogs with ET-1 (10(-9) to 10(-7) M)) increased positive staining for proliferation cell nuclear antigen (PCNA) in lung parenchyma. PCNA staining was not decreased by the endothelin-A antagonist BQ123 (10(-6) M). In addition, immunostaining for endothelin-B receptors was present in sections of unoperated but not rejecting lungs. PCNA staining in lung cultures from rejecting allotransplanted dogs was significantly gre...Continue Reading

References

Aug 1, 1976·Environmental Health Perspectives·B P LaneE J Drummond
Apr 10, 1992·European Journal of Pharmacology·E H OhlsteinC P Sung
Aug 1, 1992·British Journal of Pharmacology·C AdvenierE Naline
Dec 1, 1990·The Journal of Experimental Medicine·H EhrenreichA S Fauci
Jan 1, 1991·Journal of Cardiovascular Pharmacology·W C SessaJ R Vane
Feb 1, 1992·Proceedings of the Society for Experimental Biology and Medicine·P M HassounB L Fanburg
Oct 13, 1991·The New England Journal of Medicine·A LermanJ C Burnett
Jan 31, 1991·Biochemical and Biophysical Research Communications·W C SessaJ R Vane
Feb 1, 1990·The Journal of Clinical Investigation·C Boulanger, T F Lüscher
Aug 1, 1989·Atherosclerosis·Y HirataF Marumo
Jan 1, 1993·Journal of Cardiovascular Pharmacology·S A Douglas, E H Ohlstein
Jan 1, 1995·Circulation·A J McLartyE L Ritman
Jan 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·H SchersténF N Nilsson
Jan 1, 1996·Journal of Cardiovascular Pharmacology·P H DagassanJ P Clozel
Aug 1, 1996·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·G C Kabat
Jan 1, 1959·Experimental Cell Research·O A TROWELL

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Citations

Jul 13, 2001·Pharmacology & Therapeutics·S Naicker, K D Bhoola
Jun 12, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·François PiquardEric Epailly
Jul 9, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Hong SunAbdul S Rao
Dec 20, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Paul WexbergDietmar Glogar
Dec 22, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·M WeisW von Scheidt
Feb 8, 2000·The International Journal of Biochemistry & Cell Biology·M J BoscoeM H Yacoub
Jul 4, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H Auchincloss
May 15, 2001·Acta Physiologica Scandinavica·A BäckerH J Kramer
May 22, 1998·Journal of Cardiovascular Pharmacology·C D McDermottA Giaid
Oct 15, 1998·Transplantation·A JeppssonC G McGregor
Jun 25, 1998·American Journal of Respiratory and Critical Care Medicine·H ShennibA Giaid
Dec 26, 2001·American Journal of Respiratory Cell and Molecular Biology·Mallik R Karamsetty, James R Klinger
Jan 13, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V ShahR J Groszmann
Oct 8, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Yaron ShargallUNKNOWN ISHLT Working Group on Primary Lung Graft Dysfunction
Sep 27, 2001·Endothelium : Journal of Endothelial Cell Research·D AntoniucciL A Fitzpatrick
Jan 21, 2000·Journal of Applied Physiology·S DoiP A Murray

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