PMID: 8587461Jan 1, 1995Paper

Endothelin-1 and ETA receptor subtype are expressed in the gastric HGT-1 cell line

Journal of Cardiovascular Pharmacology
M N Mathieu, C Chevillard

Abstract

Recent studies have revealed that endothelin-1 (ET-1) may be produced by human cancer cell lines and have suggested that in vivo the peptide might play a modulatory role in the growth of stromal cells surrounding tumor cells and/or in the growth of the cancer cells themselves, through paracrine or autocrine mechanisms. Therefore, we investigated whether ET-1 and ET receptors could be expressed in the human gastric cancer cell line HGT-1. By applying the reverse transcriptase polymerase chain reaction (RT-PCR) to total RNA extracted from the cells, using oligonucleotides synthesized from the sequence of the prepro-ET-1 mRNA, we have amplified a cDNA at the expected size (453 bp), which hybridized with a labeled ET-1-specific probe. In addition, RT-PCR was carried out to test whether HGT-1 cells expressed mRNA for ETA and/or ETB receptor subtypes. The amplified products of cDNA were at the size predicted for the ETA receptor (368 bp), whereas no ETB receptor mRNA could be detected.

Citations

Sep 17, 2013·Critical Reviews in Oncology/hematology·Soussan IraniAlfred King-Yin Lam
Nov 6, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Haijuan GuBin Wang
Nov 15, 2014·Oncology Reports·Kuo-Wang TsaiHing-Chung Lam

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