PMID: 8587460Jan 1, 1995Paper

Endothelin 1 and thrombin synergistically stimulate IL-6 mRNA expression and protein production in human umbilical vein endothelial cells

Journal of Cardiovascular Pharmacology
J StankováP D'Orléans-Juste

Abstract

We investigated the effects of endothelin (ET) and thrombin on the production of interleukin 6 (IL-6) in human umbilical vein endothelial cells (HUVECs). ET-1 increased IL-6 production twofold in HUVECs incubated for 18 h with 10(-10) M ET-1. Thrombin also significantly increased IL-6 production. However, stimulation of HUVECs with ET-1 together with thrombin produced an 18-fold increase in IL-6 production. The ET-1-induced increase was concentration-dependent, with an optimal effect at 100 pM. We then examined the effect of ET-1 on IL-6 mRNA expression in thrombin-stimulated HUVECs. Unstimulated HUVECs spontaneously expressed IL-6 mRNA, which was not modulated by thrombin. Endothelin 1 upregulated IL-6 mRNA expression, with a maximal increase at 100 pM ET-1. These data indicate that the interaction of thrombin and ET-1 induces a synergistic increase in IL-6 production by HUVEC and suggest that ET-1 may therefore play a significant role in inflammation.

Citations

Feb 18, 2011·Archives of Toxicology·Mohamed Sadek Abdel-BakkyMohammad K Ashfaq
Apr 26, 2003·American Journal of Respiratory and Critical Care Medicine·Martha Sue CarrawayClaude A Piantadosi
Aug 19, 2009·Archives of Physiology and Biochemistry·Giovanni AnfossiMariella Trovati
Dec 1, 2017·International Journal of Molecular Sciences·Sean P Didion
Apr 13, 2002·Japanese Journal of Pharmacology·Richard SercombePhilippe Gomis
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·G KrishnaswamyD S Chi
Feb 24, 2006·Endocrine Reviews·Julie NigroPeter J Little
Apr 23, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Fiona A MartinPhilip M Cummins
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·K E Welty-WolfC A Piantadosi

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