PMID: 15253106Jul 16, 2004Paper

Endothelin-1 gene expression in endothelial cells is potently inhibited by a vasodilator, dilazep

Hypertension Research : Official Journal of the Japanese Society of Hypertension
Balaram ShresthaM Kawana

Abstract

Endothelin-1 (ET-1) is considered to be involved in various cardiovascular and renal disorders. The objective of this study was to investigate whether a vasodilator and antiplatelet agent, 1,4-bis[3-(3,4,5-trimethoxybenzoyloxy) propyl]perhydro-1,4-diazepine dihydrochloride monohydrate (dilazep, DZ), has an ET-1-inhibiting effect in vitro. Bovine aortic endothelial cells (BAEC) and human umbilical vein endothelial cells (HUVEC) pretreated with fetal calf serum were treated with DZ and preproET-1 (PpET-1) transcription was evaluated by Northern blot analysis. ET-1 peptide release in culture medium was evaluated by radioimmunoassay. The effect of DZ on the ET-1 promoter/enhancer apparatus was evaluated in transfection experiments using -5 kb ET-1 promoter/enhancer constructs. Modest inhibition of PpET-1 gene transcription was detected after 30 min of DZ treatment (0.56+/-0.19 vs. 1 , p<0.01) and more marked inhibition was seen at 24 h (0.04+/-0.04 vs. 1, p<0.0001). ET-1 peptide release was suppressed strongly after 3 h (382.5+/-2.9 vs. 673.5+/-74.5pg/ml, p< 0.001) and 24 h (38.8+/-9.8 vs. 5,075+/-52.0pg/ml, p<0.0001). DZ potently inhibited PpET-1 transcription in a concentration-dependent manner (0.42+/-0.18 vs. 1, p<0.001, at 100...Continue Reading

References

Oct 13, 1991·The New England Journal of Medicine·A LermanJ C Burnett
Jan 1, 1988·Journal of Clinical Pharmacology·L PoggesiM Carnovali
May 1, 1987·Pharmacological Research Communications·A CargnoniR Ferrari
Feb 1, 1980·British Journal of Pharmacology·S FujitaM Takei
Aug 1, 1995·Journal of Pediatric Gastroenterology and Nutrition·T NozueA Sako
Aug 1, 1995·Molecular and Cellular Biology·M KawanaT Quertermous
Apr 28, 2001·Hypertension Research : Official Journal of the Japanese Society of Hypertension·S IshimaruY Hirata
Jun 21, 2001·Hypertension Research : Official Journal of the Japanese Society of Hypertension·N KobayashiH Matsuoka
Jun 20, 2001·American Journal of Hypertension·E L Schiffrin
Feb 9, 2002·Kidney & Blood Pressure Research·Tsukasa NakamuraHikaru Koide

❮ Previous
Next ❯

Citations

Jan 1, 2008·Expert Review of Clinical Immunology·Paul R J AmesAnnamaria Margarita

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.