Endothelin-1 increases expression of cyclooxygenase-2 and production of interlukin-8 in hunan pulmonary epithelial cells

Peptides
Hong PengXiang Fang

Abstract

Inducible cyclooxygenase (COX-2) and inflammatory cytokines play important roles in inflammatory processes of chronic obstructive pulmonary disease (COPD). Endothelin-1 (ET-1) might be also involved in the pathophysilogical processes in COPD. In the present study, we determined whether ET-1 could regulate the expression of COX-2 and alter the production of interleukin-8 (IL-8) in human pulmonary epithelial cells (A549). Induced sputum samples were collected from 13 stable COPD patients and 14 healthy subjects. The COX-2 protein, ET-1, PGE(2) and IL-8 in these sputum samples were analyzed. A549 cells were incubated with ET-1 in the presence or absence of celecoxib, a selective COX-2 inhibitor. The expression of COX-2 protein in the cell and the amounts of PGE(2) and IL-8 in the medium were measured. The levels of COX-2 protein, ET-1, PGE(2) and IL-8 were significantly increased in induced sputum from COPD patients when compared to healthy subjects. ET-1 increased the expression of COX-2 protein, as well as the production of PGE(2) in A549 cells. Increased production of PGE(2) was inhibited by celecoxib. ET-1 also increased the production of IL-8. Interestingly, ET-1-induced production of IL-8 was also inhibited by celecoxib. The...Continue Reading

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Citations

Mar 10, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D EwenJ K Shute
Dec 3, 2013·Molecular and Cellular Endocrinology·Hong LiPeiqing Liu
Oct 26, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·R F ClaudinoG A Rae
Jun 22, 2017·Environmental Science & Technology·Ying-Hsuan LinJason D Surratt

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