Endothelin-1-induced constriction inhibits nitric-oxide-mediated dilation in isolated rat resistance arteries

Journal of Vascular Research
E N BakkerP Sipkema

Abstract

Vascular endothelial cells release dilatory compounds like nitric oxide and prostacyclin, as well as contractile factors like endothelin-1 (ET-1). We investigated the interaction of ET-1 with nitric-oxide-mediated dilation in cannulated pressurized (75 mm Hg) arterioles from rat cremaster muscle (180 +/- 3 microm). Arterioles constricted spontaneously to 101 +/- 3 microm, while ET-1 (0.4 nM) increased constriction to 78 +/- 3 microm. Acetylcholine, an endothelium-dependent nitric-oxide-mediated vasodilator induced a dose-dependent dilation during spontaneous tone. After addition of ET-1, the response to acetylcholine was significantly impaired. Nitroprusside, an endothelium-independent nitric oxide donor, induced a dose-dependent dilation that was almost completely inhibited by ET-1. In contrast, 8-Br-cGMP-induced dilation was unaffected. Thus, ET-1 appears to inhibit nitric-oxide-mediated dilation at the level of cGMP formation or degradation. The effect of ET-1 appears to be specific for nitric oxide as responses to a prostacyclin analogue were impaired at low doses only. The inhibitory effect of ET-1 on nitric-oxide-mediated dilation could be mimicked with high potassium (65 +/- 6 microm), but not with phenylephrine (74 +/- ...Continue Reading

Citations

Jul 12, 2011·Pflügers Archiv : European journal of physiology·Daryl O SchwenkeMikiyasu Shirai
Aug 24, 2002·American Journal of Obstetrics and Gynecology·Ramon G V SmoldersPieter Sipkema
Feb 21, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Robert H RosaLih Kuo
Feb 23, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y ChenM Spatz
Sep 27, 2001·Endothelium : Journal of Endothelial Cell Research·P BarbeauB Gutin
Jun 8, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikki L JerniganThomas C Resta
Dec 11, 2003·American Journal of Physiology. Endocrinology and Metabolism·Seiji MaedaMitsuo Matsuda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.