Endothelin-1 induces serine phosphorylation of the adaptor protein p66Shc and its association with 14-3-3 protein in glomerular mesangial cells

The Journal of Biological Chemistry
M FoschiA Sorokin

Abstract

Endothelin-1 (ET-1) is a vasoconstrictor peptide known to be a potent mitogen for glomerular mesangial cells (GMC). In the current study, it is demonstrated that ET-1 treatment of GMC results in serine phosphorylation of the 66-kDa isoform of the adapter protein Shc (p66(Shc)). ET-1-induced serine phosphorylation of p66(Shc) requires activation of the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signaling module and is efficiently inhibited by both a MAPK/ERK kinase (MEK)-selective inhibitor and adenovirus-mediated transfer of a dominant interfering MEK1 mutant. Furthermore, adenovirus-mediated transfer of a constitutively active MEK1 mutant was found to markedly increase p66(Shc) serine phosphorylation. Adenoviruses encoding constitutively active mutants of MAPK kinases 3 and 6 (upstream kinases of p38(MAPK)) and 7 (upstream kinase of c-Jun NH(2)-terminal kinase) failed to induce serine phosphorylation of this adaptor protein. Serine phosphorylation of p66(Shc) resulted in its association with the serine binding motif-containing protein 14-3-3. ET-1-induced phosphorylation of a serine encompassed in the 14-3-3 binding motif of p66(Shc) was confirmed in experiments employing anti-phospho-1...Continue Reading

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C M CrewsR L Erikson
Jan 1, 1990·Methods in Enzymology·M S Simonson, M J Dunn
May 1, 1996·Kidney International·D BokemeyerM J Dunn
Jan 1, 1996·Kidney International·J D SraerE Rondeau
Mar 1, 1997·Kidney International·S ArakiR Kikkawa
Mar 7, 1998·The Journal of Biological Chemistry·G R FangerR R Vaillancourt
Apr 30, 1998·Advances in Cancer Research·T S LewisN G Ahn
May 23, 1998·Annual Review of Immunology·D T Chao, S J Korsmeyer
Jul 17, 1998·Methods in Molecular Biology·C H LeeJ Kuriyan
Jan 27, 2000·Cell·T Hunter
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Sep 13, 2000·Molecular and Cellular Biology·H GuB G Neel
Oct 16, 2004·Kidney International. Supplement·Ron T GansevoortPaul E de Jong

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