Endothelin-1 mediated inhibition of the acetylcholine-activated potassium current from rabbit isolated atrial cardiomyocytes

British Journal of Pharmacology
J P SpiersB Silke

Abstract

1. Endothelin-1 is a 21 amino acid peptide with potent inotropic and chronotropic actions in the heart. Relatively little is known about the underlying electrophysiological effects of the peptide. In this study, the effects of endothelin-1 (ET-1) on the acetylcholine-activated potassium current (IK(ACh) were investigated in the absence and presence of the receptor-selective antagonists, PD155080 (ETA receptor-selective) and RES-701 (ETB receptor-selective) in rabbit atrial cardiomyocytes. 2. Cells were obtained from New Zealand White rabbits (2.5-3 kg) by enzymatic dissociation with collagenase. Potassium currents were recorded, in the presence of nifedipine (5 microM), by use of the whole cell ruptured patch-clamp technique. Following stabilization, control recordings were made with standard pulse protocols, and drugs were applied by a gravity fed microperfusion system. 3. Endothelin-1 (10 nM) alone did not affect the "steady state' potassium current. Acetylcholine (1 microM) increased (P < 0.05) the potassium current to-1321 +/- 290 pA, from a control value of -955 +/- 191 pA, at a step potential of -100 mV. Acetylcholine also increased the holding current at -40 mV from +80 +/- 9 pA to +242 +/- 38 pA, and this effect was abo...Continue Reading

References

Nov 30, 1990·Biochemical and Biophysical Research Communications·R YorikaneH Koike
Jul 31, 1991·Biochemical and Biophysical Research Communications·A SakamotoT Masaki
Apr 1, 1990·American Heart Journal·M YasudaT Takeda
Jan 23, 1990·European Journal of Pharmacology·R GalronM Sokolovsky
Nov 1, 1988·Pflügers Archiv : European journal of physiology·T IshikawaT Masaki
Jan 1, 1989·Journal of Cardiovascular Pharmacology·J G ClarkeA Maseri
Sep 1, 1989·European Journal of Pharmacology·J J ReidM J Rand
Feb 28, 1989·Biochemical and Biophysical Research Communications·C S MoravecM Bond
Nov 14, 1989·European Journal of Pharmacology·T M CocksJ Angus
Apr 1, 1984·Pflügers Archiv : European journal of physiology·M Soejima, A Noma
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Oct 1, 1995·The American Journal of Physiology·Y Kurachi
Mar 1, 1995·Journal of Cardiovascular Pharmacology·E J KelsoB Silke
Jan 1, 1995·Journal of Cardiovascular Pharmacology·G BkailyD Ménard
Jan 1, 1996·Journal of Cardiovascular Pharmacology·S KobayashiY Masuda
Sep 1, 1991·Trends in Cardiovascular Medicine·D Difrancesco

❮ Previous
Next ❯

Citations

Oct 6, 1997·British Journal of Pharmacology·H TanakaM Yoshimura
May 17, 2000·Journal of Cardiovascular Pharmacology·X Y Du, S Sorota
Sep 17, 2019·Cellular Signalling·Anne NiemeyerMarie-Cecile Kienitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.

Cardiac Electrophysiology

Cardiac electrophysiology is the study of electrical activities of the heart and includes the assessment, diagnosis, and treatment of cardiac events. Find the latest research on cardiac electrophysiology here.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.