Endothelin-1 release by erythropoietin involves calcium signaling in endothelial cells

Journal of Cardiovascular Pharmacology
R G CarliniM Rothstein

Abstract

We investigated the effects of recombinant human erythropoietin (rHuEPO) on intracellular calcium ([Ca2+]i) and whether these changes regulate both endothelin-1 (ET-1) protein release and ET-1 messenger RNA (mRNA) production in bovine pulmonary arterial endothelial cells (BPAEC). rHuEPO (3.3 U/ml) induced [Ca2+]i increases from a basal level of 54 +/- 12.2 (SE) to 147 +/- 21.1 nM (p < 0.001), in fura-2-loaded BPAEC. In the presence of nifedipine (10 microM), the increases in [Ca2+]i were significantly reduced. Furthermore, when extracellular calcium ([Ca2+]o) was reduced (200 microM), there was a significant reduction in [Ca2+]i increase after stimulation with rHuEPO. Incubation of BPAEC with rHuEPO for 4 h increased ET-1 levels in the culture supernatant from 44.7 +/- 5.3 to 85 +/- 7.6 pg/ml (p < 0.001). However, when the cells were treated with rHuEPO and nifedipine, the ET-1 levels were decreased, as compared to levels resulting from treatment with rHuEPO alone (41 +/- 6.1 vs. 85 +/- 7.6 pg/ml, p < 0.001, respectively). rHuEPO also induced a fourfold increase in the level of the preproET-1 mRNA as compared with control. PreproET-1 mRNA was diminished in the presence of nifedipine and rHuEPO and rHuEPO can increase [Ca2+]i in...Continue Reading

Citations

Jan 7, 1998·Cellular Signalling·A SchaeferH Marquardt
Jun 11, 2008·Nature Clinical Practice. Nephrology·Nosratola D Vaziri
Oct 8, 2008·Shock·Roberta d'Emmanuele di Villa BiancaRaffaella Sorrentino
Mar 11, 2004·European Journal of Biochemistry·Robert Saunders, Georgios Scheiner-Bobis
Oct 13, 2010·Annual Review of Physiology·Jeffrey L GarvinPablo A Ortiz
Aug 24, 2004·Annals of Hematology·Wolfgang Jelkmann, Klaus Wagner
Nov 28, 2012·Canadian Journal of Physiology and Pharmacology·Abdel-Aziz M HusseinAhmed A Shokeir
Jan 28, 2009·Clinical and Experimental Hypertension : CHE·Hugo W HuismanNicolass T Malan
Aug 25, 2010·Pharmacology & Therapeutics·L VellyP Pisano
Sep 12, 2007·Clinical Transplantation·Suja NagarajanMinnie M Sarwal
May 20, 1999·Current Problems in Pediatrics·S E Juul
Sep 15, 2000·Clinics in Perinatology·S E Juul
Aug 24, 2002·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Richard D Semba, Sandra E Juul
Aug 12, 2016·Physiology·Kristin A Gerhold, Martin A Schwartz
Mar 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Claudio ContaldoGuido A Wanner
Jan 24, 1998·The Journal of Biological Chemistry·C LiuC T Noguchi
Sep 13, 2005·Renal Failure·Vasilios LiakopoulosIoannis Stefanidis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.