Endothelin-1 stimulates tyrosine phosphorylation of p125 focal adhesion kinase in mesangial cells.

Journal of the American Society of Nephrology : JASN
M HanedaY Shigeta

Abstract

Endothelin-1 (ET-1) is known to induce the contraction and proliferation of glomerular mesangial cells. Because ET-1 was found to stimulate the tyrosine phosphorylation of unidentified cellular proteins in cultured mesangial cells, protein tyrosine kinase might serve as one of the important signals leading to various functions of ET-1. Focal adhesion kinase (p125FAK) is a newly identified cytoplasmic protein tyrosine kinase that is activated by the phosphorylation of its own tyrosine residue. Because p125FAK was found to play a role in the signal transduction of not only integrins but also various neurotransmitters, including bombesin, endothelin, and vasopressin in Swiss 3T3 cells and Rat-1 fibroblasts, whether ET-1 could stimulate the tyrosine phosphorylation of p125FAK in glomerular mesangial cells was examined. ET-1 stimulated the tyrosine phosphorylation of p125FAK by threefold to fourfold in cultured mesangial cells. This effect of ET-1 was detected at 1 min and reached a maximum within 5 min and was blocked by BQ-123, an antagonist for ETA receptor. A23187, a calcium ionophore, failed to stimulate the tyrosine phosphorylation of p125FAK, and ET-1 was able to stimulate the tyrosine phosphorylation of p125FAK, even in a ca...Continue Reading

Citations

Jun 18, 1999·Transplantation Proceedings·L C Paul
Jul 13, 1999·Neurochemistry International·M J PérezA M Martínez
Dec 1, 1999·Transplantation Proceedings·L C Paul
Jun 26, 2001·Journal of the American Society of Nephrology : JASN·Toshiro SugimotoRyuichi Kikkawa
Sep 19, 2001·The Journal of Biological Chemistry·E S GilmoreS L Milgram
Mar 1, 1997·Kidney International·S ArakiR Kikkawa
May 1, 1997·Kidney International·S GauerR B Sterzel

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