Endothelin antagonism: effects of FP receptor agonists prostaglandin F2alpha and fluprostenol on trabecular meshwork contractility

Investigative Ophthalmology & Visual Science
H ThiemeRita Rosenthal

Abstract

This study analyzes additional mechanisms behind the ocular hypotensive effect of prostaglandin F (PGF) receptor (FP receptor) agonists PGF2alpha and fluprostenol (fluprostenol-isopropyl ester [travoprost]), which reduce intraocular pressure (IOP) in patients with glaucoma probably by enhancing uveoscleral flow. The trabecular meshwork (TM) is actively involved in IOP regulation through contractile mechanisms. Contractility of TM is induced by endothelin (ET)-1, a possible pathogenic factor in glaucoma. The involvement of FP receptor agonists in the ET-1 effects on TM function was studied. The effects of FP receptor agonists on contractility of bovine TM (BTM) were investigated using a force-length transducer. The effects of PGF2alpha on intracellular Ca2+ ([Ca2+]i) mobilization in cultured cells were measured using fura-2AM. The expression of the FP receptor protein was examined using Western blot analysis. The ET-1-induced (10(-8) M) contraction in isolated BTM was inhibited by PGF2alpha (10(-6) M) and fluprostenol (10(-6) M). This effect was blocked by FP receptor antagonists. Carbachol-induced contraction or baseline tension was not affected by PGF2alpha or fluprostenol. In cultured TM cells, ET-1 caused a transient increas...Continue Reading

Citations

Dec 18, 2013·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·H ThiemeC Schuart
Apr 11, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Giulia RenieriHagen Thieme
Mar 8, 2007·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Najam A SharifCurtis R Kelly
Oct 19, 2007·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Warren J Scherer
Sep 7, 2007·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Geoffrey R OwenStella M Robertson
Jun 27, 2007·Investigative Ophthalmology & Visual Science·Wei XueTeresa Borrás
Dec 24, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Nelson S Winkler, Michael P Fautsch
May 12, 2010·Expert Opinion on Therapeutic Targets·Travis J Good, Malik Y Kahook
May 4, 2011·Expert Opinion on Investigational Drugs·Paolo Fogagnolo, Luca Rossetti
Mar 1, 2011·Expert Opinion on Emerging Drugs·Anne J Lee, Ivan Goldberg
Dec 23, 2010·Current Eye Research·Rong-Fang WangOvidiu C Baltatu
Sep 21, 2013·Translational Vision Science & Technology·Alex L C TamYeni H Yücel
Aug 3, 2007·Experimental Eye Research·Rita RosenthalHagen Thieme
Nov 9, 2007·American Journal of Ophthalmology·Cindy K BahlerDouglas H Johnson
Sep 28, 2017·Expert Opinion on Therapeutic Targets·Jeffrey O'CallaghanPete Humphries
Apr 19, 2018·British Journal of Pharmacology·Francesco ImpagnatielloMichael V W Bergamini
Apr 22, 2018·British Journal of Pharmacology·Najam A Sharif, Peter G Klimko
Jan 1, 2008·Expert Review of Ophthalmology·Zhou WanW Daniel Stamer
Nov 9, 2010·British Journal of Pharmacology·Rita Rosenthal, Michael Fromm
Feb 14, 2020·Journal of Cellular and Molecular Medicine·Yingying ZhaoYing Ying
Apr 4, 2020·Vestnik oftalmologii·N B ChesnokovaA V Grigoryev
Jul 31, 2008·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Kay-Oliver Arend, Thomas Raber
Jan 13, 2021·Klinische Monatsblätter für Augenheilkunde·Christian van Oterendorp
Dec 17, 2008·Survey of Ophthalmology·Carol B TorisPaul L Kaufman
Mar 18, 2009·European Journal of Pharmacology·Andrea Emilse ErrastiRodolfo Pedro Rothlin

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