PMID: 9426289Feb 28, 1998Paper

Endothelin-B receptor-mediated Ca2+ signaling in human melanocytes

Pflügers Archiv : European journal of physiology
H Y KangC Lee

Abstract

The mechanism of an endothelin-1- (ET-1-) induced intracellular Ca2+ ([Ca2+]i) increase and the receptor subtype(s) responsible for this effect in single human melanocytes were studied using fura-2/AM. ET-1 induced a transient increase in [Ca2+]i in a concentration-dependent manner. The transient [Ca2+]i increase was followed by a sustained plateau level of [Ca2+]i which was higher than the initial [Ca2+]i level. IRL-1620, a specific ET-B receptor agonist, increased [Ca2+]i in a dose-dependent manner. BQ-788, a specific ET-B receptor antagonist, abolished the ET-1-induced [Ca2+]i increase, but BQ-123, a specific ET-A receptor antagonist, failed to prevent it. U73122, an inhibitor of phospholipase C (PLC), inhibited the ET-1-induced [Ca2+]i rise in a dose-dependent manner. Prior depletion of intracellular Ca2+ stores with thapsigargin, an inhibitor of Ca2+-ATPase of the endoplasmic reticulum, abolished the ET-1-induced Ca2+ transient, whereas removal of extracellular Ca2+ with EGTA eliminated the sustained rise. These results suggest that in cultured human melanocytes the binding of ET-1 to ET-B receptors and the subsequent activation of PLC mediate ET-1-induced [Ca2+]i increase. The transient [Ca2+]i increase is attributed to m...Continue Reading

Citations

Jul 31, 2003·Cell Calcium·Mansim OkaforNicholas A Delamere
Jul 25, 2009·Pigment Cell & Melanoma Research·Catherine D Van RaamsdonkShosuke Ito
Aug 22, 2002·The Journal of Clinical Investigation·Sumayah Jamal, Robert J Schneider
Apr 5, 2013·PloS One·Mugdha DeoCatherine D Van Raamsdonk
Feb 10, 2012·The Journal of Investigative Dermatology·Hedwig StaniszIvan Bogeski
Feb 14, 2017·Pigment Cell & Melanoma Research·Stuart Gordon JarrettJohn August D'Orazio
Sep 5, 2009·Chronobiology International·Fernanda Pizão FarhatAna Maria De Lauro Castrucci
Dec 11, 2019·The Journal of Cell Biology·Rachel L Belote, Sanford M Simon
Apr 14, 2020·Environmental and Molecular Mutagenesis·Soumyadeep Sarkar, Shobhan Gaddameedhi
May 27, 2020·Pigment Cell & Melanoma Research·Fagun JainCatherine D Van Raamsdonk

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