Endothelin ETA receptor expression in human cerebrovascular smooth muscle cells

British Journal of Pharmacology
J C YuA P Davenport

Abstract

1. Endothelin (ET) has been implicated in cerebrovasospasm for example, following subarachnoid haemorrhage, and blocking the interaction of ET with its receptors on cerebral vessels, may be of therapeutic benefit. The aim of our study was to characterize endothelin receptor sub-types on medial smooth muscle cells of human cerebral vessels. Cultures of vascular smooth muscle cells were explanted from human cerebral resistance vessels and characterized as human brain smooth muscle cells (HBSMCs). 2. Over a 48 h incubation period, HBSMC cultures secreted comparable levels of immunoreactive (IR) big endothelin-1 (big ET-1) and IR endothelin (ET): 12.7 +/- 10.3 and 8.3 +/- 5.6 pmol/10(6) cells, respectively (mean +/- s.e. mean from three different individuals), into the culture medium. 3. Total RNA was extracted from cultures of human brain smooth muscle cells. Reverse-transcriptase polymerase chain reaction (RI-PCR) assays and subsequent product separation by agarose gel electrophoresis revealed single bands corresponding to the expected product sizes encoding cDNA for ETA (299 base pairs) and ETB (428 base pairs) (n = 3 different cultures). 4. Autoradiography demonstrated the presence of specific binding sites for [125I]-ET-1 whic...Continue Reading

References

Oct 1, 1992·Stroke; a Journal of Cerebral Circulation·W I Rosenblum
Jan 1, 1992·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H EhrenreichF D Goebel
Aug 1, 1992·Journal of Neurosurgery·E H Ohlstein, B L Storer
Aug 1, 1991·Stroke; a Journal of Cerebral Circulation·R L Macdonald, B K Weir
Feb 3, 1990·Lancet·H LevesqueP Creissard
Feb 1, 1990·The Journal of Clinical Investigation·C Boulanger, T F Lüscher
Jun 1, 1990·Acta Neurologica Scandinavica·H SuzukiS Shimoda
Sep 1, 1990·Neurosurgery·H KobayashiH Kawano
Feb 5, 1990·Brain Research·S YoshimotoS Murota
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Aug 1, 1983·Computer Programs in Biomedicine·G A McPherson
Jan 1, 1993·Journal of Cardiovascular Pharmacology·I ViossatP Braquet
May 1, 1994·Trends in Pharmacological Sciences·T Godfraind
Sep 1, 1994·British Journal of Pharmacology·J J MaguireA P Davenport
Sep 1, 1994·Journal of the Autonomic Nervous System·M AdnerL Edvinsson
Jan 1, 1995·British Journal of Pharmacology·M G Peter, A P Davenport
Jan 1, 1995·British Journal of Pharmacology·J C Yu, A P Davenport
Jan 1, 1994·British Journal of Pharmacology·A P DavenportA M Doherty
Jan 1, 1994·Trends in Pharmacological Sciences·A P Davenport, J J Maguire
Feb 26, 1993·Biochemical and Biophysical Research Communications·D B StanimirovicM Spatz

❮ Previous
Next ❯

Citations

Jan 1, 1997·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·G I FegerM Wahl
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Katsuhito YasunoMurat Günel
Dec 18, 2001·Journal of Cardiovascular Pharmacology·Alan R ConantAlec W M Simpson
Aug 29, 2002·Journal of Cardiovascular Pharmacology·Yoshifumi KawanabeTomoh Masaki
Dec 26, 2008·Neurological Research·Eric Michael DeshaiesA John Popp
Oct 13, 2012·Journal of the Neurological Sciences·Jiayin MiaoYannan Fang
Mar 10, 2016·Pharmacological Reviews·Anthony P DavenportJanet J Maguire
Jun 2, 2005·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Joseph F Clark, Gail Pyne-Geithman
May 2, 2014·Life Sciences·Brandi D FreemanMahalia S Desruisseaux
Feb 11, 1998·Physiological Reviews·F M Faraci, D D Heistad
Mar 3, 2019·Journal of Clinical Medicine·Eun Pyo HongJin Pyeong Jeon
Nov 14, 2020·Scientific Reports·Jie ZhuYue-Qing Huang
May 1, 2021·International Journal of Molecular Sciences·Yutaka Koyama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.