Endothelin-induced contractions of tracheal smooth muscle and identification of specific endothelin binding sites in the trachea of the rat.

British Journal of Pharmacology
N C TurnerC T Dollery

Abstract

1. The presence of specific binding sites and the contractile activity of the novel peptide, endothelin have been investigated in rat trachea. 2. Endothelin (10(-8)-10(-5) M) induced long-lasting contraction of rat tracheal rings superfused with Krebs solution (EC50 5.4 x 10(-6) M). Contractions of the tissue to 10(-6) M endothelin were attenuated in Ca2+-free medium containing 0.1 mM EGTA but unaffected by nicardipine (10(-7) M). 3. After equilibration in Ca2+-free medium (without EGTA) a return to normal Ca2+ concentrations (2.5 mM), 30 min or 60 min following endothelin (10(-6) M), produced a sustained contraction of the tissue. 4. Specific binding sites for endothelin were identified on rat tracheal smooth muscle (KD 1.34 x 10(-10) M, maximal binding 1.2 fmol mm-2). Specific binding sites were also identified on nerve trunks. Endothelin binding was unaffected by co-incubation with nicardipine (10(-7) M) or verapamil (10(-7) M). 5. The discrepancy between the apparent KD for endothelin binding and the EC50 for endothelin-induced contraction suggests that the endothelin binding sites identified in this study may not be associated with the receptors mediating contraction. 6. These results indicate that endothelin binding sites...Continue Reading

References

Oct 1, 1986·British Journal of Pharmacology·S L AllenR C Small
Jan 1, 1985·British Journal of Clinical Pharmacology·I W Rodger
Aug 15, 1988·Biochemical and Biophysical Research Communications·Y HirataS Sakakibara
Sep 13, 1988·European Journal of Pharmacology·Y UchidaS Hasegawa
Oct 1, 1984·British Journal of Pharmacology·D Raeburn, I W Rodger
Jul 1, 1984·British Journal of Pharmacology·C AdvenierA Renier

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Citations

Apr 1, 1990·Neurochemical Research·W Lovenberg, R C Miller
Oct 23, 1990·European Journal of Pharmacology·B SarriáC Advenier
Mar 1, 1990·Trends in Pharmacological Sciences·W G Nayler
Dec 15, 1994·European Journal of Pharmacology·J E NallyJ C McGrath
Jan 1, 1992·British Journal of Pharmacology·P J HenryR G Goldie
Aug 1, 1992·British Journal of Pharmacology·C AdvenierE Naline
Sep 1, 1993·British Journal of Pharmacology·D W HayB J Undem
Sep 22, 1990·BMJ : British Medical Journal·T J Hamblin
Aug 2, 2000·American Journal of Respiratory Cell and Molecular Biology·H OonumaM Omata
Sep 1, 1992·Pulmonary Pharmacology·F PonsP Braquet
Nov 1, 1996·Angiology·J I BrodyG B Fink
Jan 30, 1999·Clinical Pediatrics·A R O'HaganA Arroliga
Apr 9, 2008·Kidney & Blood Pressure Research·Thomas QuaschningBerthold Hocher
Jan 1, 1990·The Journal of Pathology·N RozengurtJ M Polak
Sep 22, 1990·BMJ : British Medical Journal·R Plail
Dec 1, 1994·Journal of Receptor Research·C M YangY L Yo
Apr 1, 1997·Anesthesia and Analgesia·T SatoS F Rabito
Mar 18, 2003·Equine Veterinary Journal·A E M BenamouR Lekeux
Jan 1, 1995·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·W Y ChenJ Y Wang
Jul 14, 1999·Life Sciences·R G Goldie, P J Henry

❮ Previous
Next ❯

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