Endothelin stimulates short circuit current in a cultured epithelium.

British Journal of Pharmacology
P Y WongS J Huang

Abstract

1. Endothelin, a novel potent vasoconstrictor peptide produced by vascular endothelial cells stimulated anion secretion by a cultured secretory epithelium derived from the rat epididymis as measured by changes in short-circuit current (SCC). 2. Stimulation of the SCC was observed when endothelin was added to the basolateral or the apical side of the epithelium. The response to basolateral application was greater than that to apical application. The EC50 values were found to be 1.3 nM and 3.0 nM for basolateral and apical application, respectively. These values were about half an order to one order of magnitude higher than that required for its vasoconstrictor action. 3. The stimulation of SCC by endothelin was accompanied by a rise in the intracellular adenosine 3':5'-cyclic monophosphate (cyclic AMP) content in epididymal monolayers. 4. The effect of endothelin on SCC was mediated through an increase in prostaglandin synthesis by the tissues and was not blocked by an antagonist of angiotensin II (Sar1 Ile8 angiotensin II) or of adrenaline (propranolol). 5. It is speculated that endogenous endothelin plays an important role in the control of water and electrolyte transport in the epididymis.

References

Apr 1, 1989·British Journal of Pharmacology·C A MaggiA Meli
Jan 1, 1987·Blood Vessels·P M Vanhoutte
Sep 1, 1986·The Journal of Physiology·A W Cuthbert, P Y Wong
Jul 1, 1985·The Journal of Physiology·G M Rubanyi, P M Vanhoutte
Apr 1, 1982·British Journal of Pharmacology·A W Cuthbert, H S Margolius

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Citations

Jun 1, 1992·Pflügers Archiv : European journal of physiology·M RodenK Turnheim
Apr 27, 2005·The Journal of General Physiology·King-Ho CheungPatrick Y D Wong
Jul 30, 2020·American Journal of Physiology. Cell Physiology·Dong-Dong GaoWen-Liang Zhou
Sep 1, 1992·Pulmonary Pharmacology·S J MarciniakA P Davenport

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