Endothelium and vascular smooth muscle function in internal mammary artery after cryopreservation

The Journal of Surgical Research
G H AlmassiG N Olinger

Abstract

An optimal cryopreserved arterial conduit should have anatomic and physiologic characteristics similar to those of the fresh artery. We have cryopreserved canine internal mammary artery (IMA) with intact, prostacyclin (PGI2)-producing endothelial cells, but the underlying vascular smooth muscle appeared nonfunctional. Thus the aim of this study was to evaluate which steps of the cryopreservation method compromise vascular smooth muscle function in cryopreserved IMA. Isometric tension recording responses to vasoconstrictor agonists were used to evaluate the level of vascular muscle integrity, while endothelial function was assessed by relaxation responses to acetylcholine and by PGI2 production. These variables were measured in vessels rewarmed to 37 degrees C, following initial exposure to different stages of an eight-step computerized cryopreservation protocol (n = 10). In this protocol, tissue temperature was lowered to -10 degrees C (steps 1-2), rapidly reduced to -12 degrees C (steps 3-4), reduced further to -40 degrees C at a rate of -0.5 degrees C/min (steps 5-6), lowered to -70 degrees C (step 7), and finally equilibrated at - 196 degrees C by immersion in liquid nitrogen (step 8). Compared to fresh IMA, the amplitude of...Continue Reading

Citations

Sep 26, 2006·Medical Engineering & Physics·Paul S SteifYoed Rabin
Jan 24, 2006·The Journal of Surgical Research·Peitao WangXing Li Wang
Aug 27, 1998·Journal of Vascular Surgery·F StankeP Devillier
Aug 9, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Daniela NegriniAndrea Moriondo
Nov 26, 2009·Clinical and Experimental Pharmacology & Physiology·Dirk F van HeldenMohammad S Imtiaz
Aug 7, 2002·British Journal of Pharmacology·James L R Fox, Pierre-Yves von der Weid

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