PMID: 8456988Mar 1, 1993Paper

Endothelium-dependent contractions to oxygen-derived free radicals in the canine basilar artery

The American Journal of Physiology
Z S KatusicP M Vanhoutte

Abstract

Experiments were designed to determine the effect of oxygen-derived free radicals in isolated canine basilar arteries. Rings with and without endothelium were suspended for isometric tension recording in modified Krebs-Ringer bicarbonate solution bubbled with 95% O2-5% CO2 (temperature = 37 degrees C; pH = 7.4). A radioimmunoassay technique was used to measure production of prostaglandins and thromboxane B2. Xanthine oxidase (1-9 mU/ml, in the presence of 10(-4) M xanthine) and hydrogen peroxide (10(-6) to 10(-4) M) caused concentration-dependent contractions. The removal of endothelium reversed these contractions into relaxations. Contractions to xanthine oxidase and hydrogen peroxide were inhibited in the presence of superoxide dismutase (150 U/ml), catalase (1,200 U/ml), indomethacin (10(-5) M), and SQ 29548 (10(-6) M) but not in the presence of deferoxamine (10(-4) to 10(-3) M) and dimethyl sulfoxide (10(-4) M). NG-monomethyl-L-arginine (3 x 10(-5) M) augmented the contractions to hydrogen peroxide. Xanthine oxidase stimulated production of 6-ketoprostaglandin F1 alpha, prostaglandin F2 alpha, prostaglandin E2, and thromboxane B2. The stimulatory effect was prevented by the removal of endothelial cells. These studies sugges...Continue Reading

Citations

Oct 1, 1995·Circulation Research·C M TanR D Feldman
Feb 14, 2002·Journal of Applied Physiology·Zhi-Chao LiDe-Hua Zhao
Oct 18, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaohua WangYuansheng Gao
Jul 18, 2008·The Journal of Pharmacology and Experimental Therapeutics·Eva H C Tang, Paul M Vanhoutte
Sep 30, 2016·Platelets·Stephanie A HoustonSimon Kennedy
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Jun 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Anna K BrzezinskaWilliam M Chilian
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Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Sean P Didion, Frank M Faraci
May 6, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Nina L TsakadzeStanley E D'Souza
Nov 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Naris Thengchaisri, Lih Kuo
Jan 20, 2006·Journal of Applied Physiology·Frank M Faraci

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