Endothelium-dependent relaxation of rat mesenteric arterial rings by a Phoneutria nigriventer venom fraction

Japanese Journal of Pharmacology
MariaLourdesDuarte WeinbergC R Diniz

Abstract

Phoneutria nigriventer spider venom has been described as acting on several cardiovascular sites. In the present paper, a semi-purified fraction of this spider venom was studied to observe any contractile or relaxing effect in rat mesenteric arterial rings (MAR). Spider venom was first fractionated by gel filtration and subsequently by gradual isocratic steps in 0.1% trifluoroacetic acid. The first fraction of this last fractionation step is studied in the present paper and due to its main effect, it was named NORF (nitric oxide releasing fraction). No direct contractile effect was induced by NORF in relaxed MAR, suggesting no NORF-induced neurotransmitter release in this preparation. No significant influence of NORF was observed on concentration-response curves to phenylephrine on endothelium-denuded MAR, but a significant inhibitory shift of concentration-respense curves was observed on endothelium-preserved MAR (EC50 = 0.39 +/- 0.07 microM for control and EC50 = 0.68 +/- 0.14 microM with NORF). NORF induced concentration-dependent relaxation in endothelium-preserved phenylephrine pre-contracted MAR but not in endothelium-denuded MAR. NORF-induced relaxation was inhibited by the nitric oxide synthase inhibitor L-NAME (N(omega...Continue Reading

References

Sep 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·E AntunesG de Nucci
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·L Rezende JúniorC R Diniz
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Apr 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·S MarangoniG de Nucci
May 1, 1997·Journal of Vascular Research·C M Boulanger, P M Vanhoutte

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Citations

Nov 10, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M RichardsonM N Cordeiro
Jun 25, 2013·Toxicon : Official Journal of the International Society on Toxinology·C C HortaE Kalapothakis
Mar 19, 2019·Toxicon : Official Journal of the International Society on Toxinology·Luis Fernando Díaz-PeñaAlejandro García-Arredondo

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