Endothelium-derived microparticles from chronically thromboembolic pulmonary hypertensive patients facilitate endothelial angiogenesis

Journal of Biomedical Science
Daria BelikBeata Wojciak-Stothard

Abstract

Increased circulating levels of endoglin(+) endothelial microparticles (EMPs) have been identified in several cardiovascular disorders, related to severity. Endoglin is an auxilary receptor for transforming growth factor β (TGF-β) important in the regulation of vascular structure. We quantified the number of microparticles in plasma of six patients with chronic thromboembolic pulmonary hypertension (CTEPH) and age- and sex-matched pulmonary embolic (PE) and healthy controls and investigated the role of microparticle endoglin in the regulation of pulmonary endothelial function in vitro. Results show significantly increased levels of endoglin(+) EMPs in CTEPH plasma, compared to healthy and disease controls. Co-culture of human pulmonary endothelial cells with CTEPH microparticles increased intracellular levels of endoglin and enhanced TGF-β-induced angiogenesis and Smad1,5,8 phosphorylation in cells, without affecting BMPRII expression. In an in vitro model, we generated endothelium-derived MPs with enforced membrane localization of endoglin. Co-culture of these MPs with endothelial cells increased cellular endoglin content, improved cell survival and stimulated angiogenesis in a manner similar to the effects induced by overexpr...Continue Reading

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Citations

Apr 10, 2017·Angiogenesis·Mays MerjanehVéronique J Moulin
Jul 26, 2017·Nature Reviews. Urology·UNKNOWN Young Academic Urologists Men's Health GroupMaarten Albersen
Jan 11, 2018·Journal of Cellular Physiology·Alexandra LabergeVéronique J Moulin
Jan 10, 2018·Frontiers in Physiology·Jie ChenPinhua Pan
Jan 13, 2018·Frontiers in Cardiovascular Medicine·Lina BadimonTeresa Padró
May 29, 2018·Journal of Cellular and Molecular Medicine·Fan DengLiangqing Zhang
Jun 21, 2018·American Journal of Respiratory Cell and Molecular Biology·Katharina JandlGrazyna Kwapiszewska
Apr 23, 2019·Annals of Medicine·Christos VoukalisGregory Y H Lip
Jul 10, 2020·Respiratory Research·Aradhana MohanNavneet K Dhillon
Aug 20, 2021·Current Cardiology Reports·George A AlbaSudarshan Rajagopal

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Methods Mentioned

BETA
flow cytometry
light scattering
ELISA
transfection
immunoprecipitation
electrophoresis
Assay
Confocal microscopy

Software Mentioned

pDisplay
GraphPad Prism
Nanoparticle Tracking Analysis ( NTA )
Image J

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