Endothelium-Targeted Deletion of microRNA-15a/16-1 Promotes Poststroke Angiogenesis and Improves Long-Term Neurological Recovery.

Circulation Research
Ping SunKe-Jie Yin

Abstract

Angiogenesis promotes neurological recovery after stroke and is associated with longer survival of stroke patients. Cerebral angiogenesis is tightly controlled by certain microRNAs (miRs), such as the miR-15a/16-1 cluster, among others. However, the function of the miR-15a/16-1 cluster in endothelium on postischemic cerebral angiogenesis is not known. To investigate the functional significance and molecular mechanism of endothelial miR-15a/16-1 cluster on angiogenesis in the ischemic brain. Endothelial cell-selective miR-15a/16-1 conditional knockout (EC-miR-15a/16-1 cKO) mice and wild-type littermate controls were subjected to 1 hour middle cerebral artery occlusion followed by 28-day reperfusion. Deletion of miR-15a/16-1 cluster in endothelium attenuates post-stroke brain infarction and atrophy and improves the long-term sensorimotor and cognitive recovery against ischemic stroke. Endothelium-targeted deletion of the miR-15a/16-1 cluster also enhances post-stroke angiogenesis by promoting vascular remodeling and stimulating the generation of newly formed functional vessels, and increases the ipsilateral cerebral blood flow. Endothelial cell-selective deletion of the miR-15a/16-1 cluster up-regulated the protein expression of ...Continue Reading

References

Sep 1, 1994·Stroke; a Journal of Cerebral Circulation·J KrupinskiJ M Wang
Sep 18, 1993·Lancet·J KrupinskiS Kumar
Jan 28, 1998·Stroke; a Journal of Cerebral Circulation·N J AlkayedP D Hurn
Aug 13, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T HayashiY Itoyama
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·U E KniesM Clauss
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K L JinD A Greenberg
May 15, 2001·American Journal of Physiology. Cell Physiology·I Zachary
Feb 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Takeshi HayashiPak H Chan
Dec 16, 2005·Nature·Peter Carmeliet
Dec 17, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shauna M Dauphinee, Aly Karsan
Jan 7, 2006·Nature Medicine·Benoit R Gauthier, Claes B Wollheim
Feb 2, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jackelyn A AlvaM Luisa Iruela-Arispe
Feb 24, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ke-Jie YinJin-Moo Lee
Mar 31, 2007·Circulation Research·Laurent LamaliceJacques Huot
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·George A CalinCarlo M Croce
May 15, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Chien-Yuan LinTeng-Nan Lin
Nov 27, 2008·Journal of Cellular Physiology·Yujing BiRuifu Yang
Jan 15, 2009·Acta Neuropathologica·Heike Beck, Karl H Plate
Feb 28, 2009·Stroke; a Journal of Cerebral Circulation·Marc FisherUNKNOWN STAIR Group
Feb 28, 2009·Nature Reviews. Drug Discovery·Andrew Beenken, Moosa Mohammadi
Apr 21, 2009·Lancet Neurology·Zheng Gang Zhang, Michael Chopp
Jun 27, 2009·Science·Beverly A Purnell, Pamela J Hines
Nov 6, 2009·PloS One·Kay Sin TanKandiah Jeyaseelan
Jan 8, 2010·Neurobiology of Disease·Ke-Jie YinY Eugene Chen
Mar 13, 2010·Nature Protocols·Irina Arnaoutova, Hynda K Kleinman
May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ke-Jie YinY Eugene Chen
Jun 14, 2012·The Journal of Biological Chemistry·Ke-Jie YinY Eugene Chen
Feb 15, 2013·Brain : a Journal of Neurology·Ke-Jie YinY Eugene Chen
Sep 6, 2013·The Journal of Antimicrobial Chemotherapy·Wei LiuUNKNOWN China-SCAN team
Dec 3, 2013·Progress in Neurobiology·Jialing LiuGuo-Yuan Yang

❮ Previous
Next ❯

Citations

Jan 5, 2021·Frontiers in Cell and Developmental Biology·Bo DouXixi Yin
Feb 23, 2021·Frontiers in Molecular Neuroscience·Li GanShixiong Deng
Mar 14, 2021·Translational Stroke Research·Xuejing ZhangJane Q Wang
Mar 14, 2021·The American Journal of Pathology·Julianne W Ashby, Julia J Mack
Mar 30, 2021·Frontiers in Pediatrics·Salvatore TagliaferriGiuseppe Pignataro
Apr 28, 2021·Neurochemistry International·Jian ChenYun Xu
Apr 30, 2021·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ping SunKe-Jie Yin

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
genotyping
PCR

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.