PMID: 2092361Nov 1, 1990Paper

Endotoxic activity in faeces of mice from different microbiological environments

Research in Microbiology
S KawamuraT Mitsuoka

Abstract

Chromogenic Limulus amoebocyte lysate (LAL) tests were evaluated for measuring endotoxic activity in faeces of mice from different microbiological environments. Faeces of BALB/c mice from germ-free (GF), specific-pathogen-free (SPF) and conventional (CV) colonies had no inhibitory activity on the test reaction. GF and SPF faeces had heat-labile non-specific amidase activity which gave false-positive reaction, while CV did not. GF faeces had a heat-stable non-endotoxic effect on the LAL system. Average endotoxin levels in faeces of GF, SPF and CV mice were 0.2, 0.8 and 17 to 24 micrograms/g wet weight, respectively. Results suggest that the microbiological status of mice influences not only the faecal endotoxin level but also the specificity of the test reaction.

References

Jan 1, 1978·Thrombosis Research·R B FriedmanM Szczecinski
Jan 1, 1986·Scandinavian Journal of Infectious Diseases·H GorisD van der Waaij
Jun 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·T ObayashiT Kawai
Jul 30, 1981·Biochemical and Biophysical Research Communications·A KakinumaY Sugino

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Citations

May 1, 1993·Journal of Reproductive Immunology·D A ClarkG Chaouat
Jun 1, 2006·Clinical Orthopaedics and Related Research·Jennifer L NalepkaEdward M Greenfield
Oct 9, 2004·BioTechniques·Jennifer L Nalepka, Edward M Greenfield

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