Endotoxic shock leads to apoptosis in vivo and reduces Bcl-2

Shock
J HaendelerS Dimmeler

Abstract

Endotoxic shock results in multiple organ failure. At present, two different mechanisms of cellular destruction are of interest: necrosis and apoptosis. Therefore, we started to investigate in pigs whether cell death due to apoptosis is involved in this pathophysiological process. DNA fragments were detected by ELISA specific for histone-associated DNA fragments in three different experimental settings, Pigs were laparotomized followed by endotoxin infusion (ETOX group, n = 6), or laparotomized without endotoxin infusion (LAP group; n = 3) and compared with control animals (n = 3). 6 h of continuous endotoxin-infusion (5 micrograms/kg/h) resulted in a significantly enhanced apoptosis in liver as compared with controls animals (295 +/- 11%; p < .01), whereas in the LAP group, only a minor increase of 166 +/- 14% was detectable. In spleen of endotoxin-treated animals, an enhanced apoptosis of 150 +/- 12% compared with controls was shown in the ETOX group (p = .02), whereas kidney remained unaffected. These results were confirmed by agarose DNA gel electrophoresis. A typical DNA ladder was detected in liver and spleen, but not in kidney of endotoxin-treated animals. Furthermore, immunohistochemical detection of DNA strand breaks w...Continue Reading

Citations

Feb 4, 2010·Apoptosis : an International Journal on Programmed Cell Death·Varisa PongrakhananonPithi Chanvorachote
May 17, 2011·Apoptosis : an International Journal on Programmed Cell Death·Sudjit LuanpitpongYon Rojanasakul
Nov 27, 2002·European Journal of Pharmacology·Manabu UemuraToshikazu Yoshikawa
Aug 28, 1998·European Journal of Pharmacology·B BrüneK B Sandau
Jun 26, 2002·Journal of the American College of Surgeons·Farnaz N MoazzamJin Wen Ding
Oct 14, 2006·Nature Reviews. Immunology·Richard S Hotchkiss, Donald W Nicholson
May 12, 2000·Critical Care Medicine·R Mahidhara, T R Billiar
Jan 25, 2003·International Anesthesiology Clinics·Avine Lydon, J A Jeevendra Martyn
Oct 16, 2003·Shock·Philip Efron, Lyle L Moldawer
Aug 14, 2001·American Journal of Respiratory and Critical Care Medicine·C AdrieB S Polla
May 8, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Shinji MorimotoTadaaki Iwasaki
Aug 14, 2001·Biochemical and Biophysical Research Communications·F JungS Dimmeler
Mar 3, 2004·Intensive Care Medicine·Rozenn Le BerreBenoit P Guery
Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S S YadavP A Clavien
Nov 5, 2003·Journal of Cellular and Molecular Medicine·Alexandrina Burlacu
Aug 23, 2011·Scandinavian Journal of Immunology·Jeppe Sylvest NielsenJan Krog
Jan 7, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Christoffer SøllingElse K Tønnesen
Oct 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alain BarrierAntoinette Lemoine
Apr 1, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T SchäferB Vollmar
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip A EfronLyle L Moldawer
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·S YasuharaJ A Martyn
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard S HotchkissIrene E Karl
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·T E McDonaldK R Walley
Sep 29, 2020·Frontiers in Immunology·Zhenxing ChengGuozheng Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.