Endotoxin-induced inflammation down-regulates L-type amino acid transporter 1 (LAT1) expression at the blood-brain barrier of male rats and mice

Fluids and Barriers of the CNS
Gábor WittmannRonald M Lechan

Abstract

We recently reported that bacterial lipopolysaccharide (LPS)-induced inflammation decreases the expression of the primary thyroid hormone transporters at the blood-brain barrier, organic anion-transporting polypeptide 1c1 (OATP1c1) and monocarboxylate transporter 8 (MCT8). L-type amino acid transporters 1 and 2 (LAT1 & LAT2) are regarded as secondary thyroid hormone transporters, and are expressed in cells of the blood-brain or blood-cerebrospinal fluid barrier and by neurons. The purpose of this study was to examine the effect of LPS-induced inflammation on the expression of LAT1 and LAT2, as these may compensate for the downregulation of OATP1c1 and MCT8. LPS (2.5 mg/kg body weight) was injected intraperitoneally to adult, male, Sprague-Dawley rats and C57Bl/6 mice, which were euthanized 2, 4, 9, 24 or 48 h later. LAT1 and LAT2 mRNA expression were studied on forebrain sections using semiquantitative radioactive in situ hybridization. LAT1 protein levels in brain vessels were studied using LAT1 immunofluorescence. Statistical comparisons were made by the non-parametric Kruskal-Wallis and Dunn's tests. In both species, LAT1 mRNA decreased in brain blood vessels as soon as 2 h after LPS injection and was virtually undetectable ...Continue Reading

References

Mar 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M MauriceA L Hubbard
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·R J BoadoW M Pardridge
May 11, 2000·The Journal of Comparative Neurology·F Mercier, G I Hatton
Sep 30, 2000·Brain Research·T KageyamaS Shimohama
Nov 18, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R DuelliL R Drewes
Dec 16, 2000·Neuroreport·T KageyamaS Shimohama
Mar 5, 2003·Journal of Neurochemistry·Ruben J BoadoWilliam M Pardridge
Apr 8, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Shinobu MoriTetsuya Terasaki
Nov 25, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bradley E Enerson, Lester R Drewes
Feb 24, 2007·The Journal of Clinical Investigation·Marija TrajkovicHeike Heuer
Sep 14, 2007·The Journal of Comparative Neurology·Maia V KokoevaJeffrey S Flier
Jan 31, 2009·Endocrinology·Heike Heuer, Theo J Visser
Jul 31, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eva K WirthUlrich Schweizer
Oct 17, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Atsushi OseYuichi Sugiyama
Mar 3, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nadine RuderischFrançois Verrey
Aug 13, 2011·Thyroid Research·Anita KinneGerd Krause
Mar 27, 2012·Nature Neuroscience·Daniel A LeeSeth Blackshaw
Sep 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Keitaro HayashiNaohiko Anzai
Apr 3, 2014·The Journal of Clinical Investigation·Steffen MayerlHeike Heuer
Jul 6, 2014·Frontiers in Endocrinology·Julia Müller, Heike Heuer
Jul 11, 2014·Frontiers in Endocrinology·Eva K WirthJosef Köhrle

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Citations

Mar 21, 2016·Brain, Behavior, and Immunity·Aravinthan Varatharaj, Ian Galea
Sep 21, 2018·The Australian and New Zealand Journal of Psychiatry·Gerwyn MorrisMichael Berk
May 17, 2019·Journal of Medical Internet Research·Onil BhattacharyyaEric C Schneider
Jun 17, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Máté DurstZsuzsanna E Tóth
May 8, 2020·Pharmaceutical Research·Elena PurisKristiina M Huttunen
Aug 10, 2016·The Journal of Comparative Neurology·Gábor WittmannRonald M Lechan
Mar 23, 2020·Brain Structure & Function·Rita MatuskaZsuzsanna E Tóth

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