Endotoxin induced peritonitis elicits monocyte immigration into the lung: implications on alveolar space inflammatory responsiveness

Respiratory Research
Mirko SteinmüllerUlrich A Maus

Abstract

Acute peritonitis developing in response to gram-negative bacterial infection is known to act as a trigger for the development of acute lung injury which is often complicated by the development of nosocomial pneumonia. We hypothesized that endotoxin-induced peritonitis provokes recruitment of monocytes into the lungs, which amplifies lung inflammatory responses to a second hit intra-alveolar challenge with endotoxin. Serum and lavage cytokines as well as bronchoalveolar lavage fluid cells were analyzed at different time points after intraperitoneal or intratracheal application of LPS. We observed that mice challenged with intraperitoneal endotoxin developed rapidly increasing serum and bronchoalveolar lavage fluid (BALF) cytokine and chemokine levels (TNFalpha, MIP-2, CCL2) and a nearly two-fold expansion of the alveolar macrophage population by 96 h, but this was not associated with the development of neutrophilic alveolitis. In contrast, expansion of the alveolar macrophage pool was not observed in CCR2-deficient mice and in wild-type mice systemically pretreated with the anti-CD18 antibody GAME-46. An intentional two-fold expansion of alveolar macrophage numbers by intratracheal CCL2 following intraperitoneal endotoxin did n...Continue Reading

References

Sep 1, 1996·American Journal of Respiratory and Critical Care Medicine·R B GoodmanT R Martin
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·W A KuzielN Maeda
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·J G WagnerR A Roth
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RosseauJ Lohmeyer
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F NomuraS Akira
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S RosseauJ Lohmeyer
Jan 3, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·U MausJ Lohmeyer
Apr 24, 2002·American Journal of Respiratory Cell and Molecular Biology·M Audrey KoayJohn W Christman
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ulrich A MausJürgen Lohmeyer
Aug 3, 2002·American Journal of Respiratory and Critical Care Medicine·Ulrich MausJürgen Lohmeyer
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ulrich A MausJürgen Lohmeyer
Nov 2, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ulrich A MausJürgen Lohmeyer

❮ Previous
Next ❯

Citations

Sep 5, 2015·PloS One·François PhilippartUNKNOWN Outcomerea study group
Oct 11, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Sanjukta ChakrabortyMariappan Muthuchamy
Feb 26, 2011·Expert Review of Respiratory Medicine·Yao ShenXiangdong Wang
Aug 11, 2012·Critical Care Medicine·François PhilippartJean-Marc Cavaillon
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hui S LeeJihee L Kang
Aug 3, 2010·Acta Cirúrgica Brasileira·Ivana Duval de AraujoValbert Nascimento Cardoso
May 24, 2017·Scientific Reports·Anupam AdhikariMartin Olivier
Jul 21, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Maciej CabanskiJürgen Lohmeyer
May 14, 2019·Frontiers in Cardiovascular Medicine·Nicola PotereStefano Toldo
Dec 17, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Yongzhi WangHenrik Thorlacius
Jun 26, 2007·Shock·J W Olivier van TillMarja A Boermeester

❮ Previous
Next ❯

Methods Mentioned

BETA
bronchoalveolar
lavage
ELISA
bronchoalveolar lavage

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.