PMID: 15223810Jun 30, 2004Paper

Endotoxin-induced uveitis in cyclooxygenase-2-deficient mice

Investigative Ophthalmology & Visual Science
Jingsheng TuoChi-Chao Chan

Abstract

Endotoxin-induced uveitis (EIU) is a model that mimics human acute anterior uveitis. Cyclooxygenase (COX)-2 is an enzyme that initiates the conversion of arachidonic acid (AA) into prostaglandins (PGs), whereas 5-lipoxygenase (5-LO) generates leukotrienes (LT). The purpose of this study was to delineate the role of COX-2 in acute ocular inflammation. EIU was induced in wild-type (WT), heterozygotic (COX-2(+/-)) and COX-2 null (COX-2(-/-)) mice by injection of lipopolysaccharide (LPS). Other mice were coinjected with LPS and IFN gamma. Ocular histology, serum cytokines, and AA products determined by ELISA, and relevant ocular messengers determined by RT-PCR were compared among the different groups. Histology showed that the EIU score was significantly enhanced in COX-2(-/-) mice in comparison to WT and COX-2(+/-). PGE(2) was increased in WT and COX-2(+/-) EIU but not in COX-2(-/-) EIU. LTB(4) in serum and ocular 5-LO transcripts were increased in COX-2(-/-) EIU mice in comparison with WT and COX-2(+/-) EIU mice. IL-6 increased, whereas IFN gamma decreased both in serum and ocular transcripts in COX-2(-/-) EIU mice in comparison with WT and COX-2(+/-). Furthermore, EIU was suppressed in mice treated with recombinant IFN gamma, as...Continue Reading

Citations

Nov 5, 2008·American Journal of Veterinary Research·María J Del SoleRuth E Rosenstein
Mar 10, 2009·Trends in Pharmacological Sciences·Sang-Ho ChoiFrancesca Bosetti
Jun 4, 2005·Clinical & Experimental Ophthalmology·Rafael IribarrenTomás Pfortner
Mar 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Qunshan Jia, James J Pestka
Nov 5, 2020·ACS Chemical Neuroscience·Navrinder KaurRamesh Chandra

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