Endotoxin liberation studied by biological and chemical methods. Chemical characterization of six meningococcal lipopolysaccharides

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
K BrynB M Andersen

Abstract

Release of endotoxin (or lipopolysaccharides, LPS) from four meningococcal strains was studied with a chemical and a biological technique. Two strains were endotoxin-liberating (E+; 270E+ and 840E+) and two had no or low endotoxin release E-; 270E- and 840E-). LPS was quantitated by gas chromatography (GC) of LPS-specific hydroxy fatty acid, in parallel with assay of endotoxin by Limulus Amebocyte Lysate (LAL), in cell suspensions of equal O.D. and in filtered samples. The GC and LAL methods showed a reasonably good agreement in the determination of LPS in filtrates, which had distinctly higher levels (approx. 10-100 times) for the E+ strains than the E- strains, in accordance with earlier LAL studies. This difference was not due to overproduction of LPS in the E+ strains, since all four strains had the same level of LPS (by GC) in cell suspensions of equal O.D. Here the agreement between the GC and LAL methods was substantially less, with lower values by LAL for the two E-strains. The chemical composition of purified LPS was determined by methanolysis and GC for the four strains and for two additional strains 247 and 714 with a high degree of genetic similarity with strains 270E- and 840E-, respectively. Amounts of unphosphory...Continue Reading

References

Jan 1, 1987·Scandinavian Journal of Infectious Diseases·B M AndersenK Bøvre
Jan 1, 1988·Scandinavian Journal of Infectious Diseases·B M Andersen, O Solberg
Apr 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·D A CaugantO Solberg
Jul 1, 1987·Microbial Pathogenesis·R K SelanderT S Whittam
Jun 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·T ObayashiT Kawai
Jan 1, 1984·Scandinavian Journal of Infectious Diseases·B M Andersen, O Solberg
Jul 1, 1984·Reviews of Infectious Diseases·O LüderitzT Shiba

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Citations

Oct 13, 2009·Health Physics·Mary Helen Barcellos-Hoff, David H Nguyen
Dec 7, 2007·Nephron. Clinical Practice·Michael J HausmannMoshe Zlotnik
Apr 4, 2002·Drugs & Aging·Carol J Fabian, Bruce F Kimler
May 16, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·M J HausmannA Douvdevani
Feb 18, 2020·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Hao YanWei Fang

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