PMID: 9529059Apr 7, 1998Paper

Endotoxin-neutralizing protein protects against endotoxin-induced endothelial barrier dysfunction

Infection and Immunity
Douglas D BannermanSimeon E Goldblum

Abstract

Bacterial lipopolysaccharide induces tyrosine phosphorylation of paxillin, actin reorganization, and opening of the transendothelial paracellular pathway through which macromoles flux. In this study, lipid A was shown to be the bioactive portion of the lipopolysaccharide molecule responsible for changes in endothelial barrier function. We then studied whether endotoxin-neutralizing protein, a recombinant peptide that is derived from Limulus antilipopolysaccharide factor and targets lipid A, could block the effects of lipopolysaccharide on protein tyrosine phosphorylation, actin organization, and movement of 14C-bovine serum albumin across bovine pulmonary artery endothelial cell monolayers. In the presence of serum, a 6-h exposure to lipopolysaccharide (10 ng/ml) increased transendothelial 14C-albumin flux compared to the simultaneous media control. Coadministration of endotoxin-neutralizing protein (> or =10 ng/ml) with lipopolysaccharide (10 ng/ml) protected against lipopolysaccharide-induced barrier dysfunction. This protection was dose dependent, conferring total protection at endotoxin-neutralizing protein/lipopolysaccharide ratios of > or =10:1. Similarly, endotoxin-neutralizing protein was capable of blocking the lipopol...Continue Reading

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Citations

May 9, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Douglas D Bannerman, Simeon E Goldblum
Dec 17, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shauna M Dauphinee, Aly Karsan
Jan 16, 2007·Analytical Biochemistry·Graciela PrianoFernando Battaglini

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